Prostate cancer microvessels: A novel method for three-dimensional reconstruction and analysis
Prostate cancer microvessels: A novel method for three-dimensional reconstruction and analysis
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DOI：
10.1002/(sici)1097-0045(19981201)37:4
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发表时间：
1998-12-01
期刊：
影响因子：
2.8
通讯作者：
Bostwick, DG
中科院分区：
文献类型：
--
作者：
Kay, PA;Robb, RA;Bostwick, DG
背景迄今为止，前列腺癌微血管的研究一直依赖于常规的二维图像从组织切片，并有没有以前的报告三维（3D）重建和分析前列腺微血管的良性或恶性标本。关于微血管的3D结构的知识将有助于确定二维（2D）测量的实用性和局限性，以及用于确定3D测量预测前列腺癌的病理阶段和患者结果的有用性。然而，必须首先证明在3D中研究微血管的能力。我们开发了一种新的方法来可视化和分析前列腺微血管在三维连续切片的前列腺标本，包括组织制备，重建成三维体积的连续组织切片，提取血管从这个数据集，和计算的几何特征。11个地区的良性和癌症组织进行了研究和比较，以验证我们的方法。良性和恶性组织的微血管和腺体成分在三维空间中一起可视化。在3D可视化中，与良性组织相关的微血管相比，与癌症相关的微血管具有更多的任意路径，增加的迂曲度，以及与腺体成分更随意的关系。在这项探索性研究中，体积长度密度作为一种定量测量方法，比简单的微血管密度更能区分良性组织和癌症。前列腺癌中的微血管比良性组织中的微血管具有更均匀的分布和更大的迂曲度。微血管的体积长度密度显示出作为前列腺癌的3D标记物的前景。(C)1998 Wiley-Liss，Inc.
BACKGROUND. Studies of prostate cancer microvessels to date have relied on routine two-dimensional images from histologic tissue sections, and there have been no previous reports of three-dimensional (3D) reconstruction and analysis of prostatic microvessels in benign or malignant specimens. Knowledge about the 3D architecture of microvessels would be useful for determining the utility and limitations of two-dimensional (2D) measures, as well as for determining the usefulness of 3D measures to predict pathologic stage and patient outcome in prostate cancer. However, the ability to study microvessels in 3D must first be demonstrated.METHODS. We developed a novel method to visualize and analyze prostate microvessels in three dimensions from serially-sectioned prostate specimens, including tissue preparation, reconstruction of serial histologic sections into 3D volumes, extraction of vessels from this data set, and calculation of geometric characteristics. Eleven regions of benign and cancer tissue were studied and compared in an effort to validate our methodology.RESULTS. Microvessels and glandular elements from benign and malignant tissue were visualized together in three dimensions. In the 3D visualizations, microvessels associated with cancer were seen to have more arbitrary pathways, increased tortuosity, and a more casual relationship with glandular elements than microvessels associated with benign tissue. A quantitative measure, the volume length density, discriminated between benign tissue and cancer better than simple microvessel density in this exploratory study.CONCLUSIONS. Microvessels in prostate cancer have a more homogeneous distribution and greater tortuosity than those in benign tissue. Volume length density of microvessels shows promise as a 3D marker in prostate cancer. (C) 1998 Wiley-Liss, Inc.