Correlation of Plasma Protein Carbonyls and C-Reactive Protein with GOLD Stage Progression in COPD Patients.
Correlation of Plasma Protein Carbonyls and C-Reactive Protein with GOLD Stage Progression in COPD Patients.
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DOI：
10.2174/1874306400903010061
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发表时间：
2009-04-14
期刊：
影响因子：
--
通讯作者：
Hicks JJ
中科院分区：
文献类型：
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作者：
Torres-Ramos YD;García-Guillen ML;Olivares-Corichi IM;Hicks JJ
氧化应激在慢性阻塞性肺疾病(COPD)的发病机制中起重要作用。为探讨慢性阻塞性肺疾病(COPD)进展与慢性炎症和氧化损伤标志物的关系，采集了14例健康志愿者(HV，n=14)和COPD稳定期患者的血样。按GOLD分期分为三组(II期34例，III期18例，IV期20例)。测定C反应蛋白(CRP)、蛋白羰基(PC)、丙二醛(MDA)、血浆过氧化脂质(SLP)和髓过氧化物酶活性(MPO)。用体外金属离子催化反应产生的丙二醛的量来测量SLPS的血浆浓度。与HV相比，慢性阻塞性肺疾病患者的PC、SLP和CPR显著增加(p<；0.001)。丙二醛浓度和MPO活性与HV组无显著差异。总之，在COPD进展过程中，氧化应激的存在，伴随着脂质和蛋白质氧化增加，导致脂质衍生的醛和直接氨基酸侧链氧化形成的总羰基逐渐显著增加，可能成为COPD进展和氧化应激损伤的一个生物标志物和独立监测。
Oxidative stress plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). To investigate the correlation between the progression of COPD and plasma biomarkers of chronic inflammation and oxidative injury, blood samples were obtained from healthy volunteers (HV, n = 14) and stabilized COPD patients. The patients were divided into three groups according to their GOLD stage (II, n = 34; III, n = 18; IV, n = 20). C-reactive protein (CRP), protein carbonyls (PC), malondialdehyde (MDA), susceptible lipoperoxidation of plasma substrates (SLPS), and myeloperoxidase activity (MPO) were measured. The plasma concentration of SLPS was measured as the amount of MDA generated by a metal ion-catalyzed reaction in vitro. PC, SLPS, and CPR were increased significantly (p < 0.001) in COPD patients when compared to HV. MDA concentrations and MPO activities were not significantly different from those of the HV group. In conclusion, increased oxidation of lipids and proteins resulting in a progressive increase in the amount of total plasma carbonyls and oxidative stress the presence of oxidative stress during COPD progression, concomitant with an increased oxidation of lipids and proteins resulting in a progressive and significant increase in the amount of total carbonyls formed from lipid-derived aldehydes and direct amino acid side chain oxidation in plasma, may serve as a biomarker and independent monitor of COPD progression and oxidative stress injury.