Information display by transcriptional enhancers
Information display by transcriptional enhancers
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DOI：
10.1242/dev.00890
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Arnosti, DN
中科院分区：
文献类型：
--
作者：
Kulkarni, MM;Arnosti, DN
转录增强子整合位置和时间信息来调节发育控制基因的复杂表达。目前的模型表明，增强子作为计算设备，从激活子和阻遏子接收多个输入，并将其解析为单个正或负信号，该信号被传输到基础转录机器。我们表明，一个简单的，紧凑的增强子是能够代表压抑和激活状态在同一时间和同一个核。这一发现表明，紧密并列的因子结合位点，位于紧凑的顺式元件内，可以独立地解释的转录机制，可能通过连续的增强子-启动子相互作用。这些结果提供了明确的证据表明，通常归因于增强子本身的计算功能实际上与基础机制共享。与增强子功能的自主计算机模型相反，增强子活性的信息显示或“公告牌”模型可以更好地描述许多发育调节的转录增强子。
Transcriptional enhancers integrate positional and temporal information to regulate the complex expression of developmentally controlled genes. Current models suggest that enhancers act as computational devices, receiving multiple inputs from activators and repressors and resolving them into a single positive or a negative signal that is transmitted to the basal transcriptional machinery. We show that a simple, compact enhancer is capable of representing both repressed and activated states at the same time and in the same nucleus. This finding suggests that closely apposed factor binding sites, situated within compact cis-elements, can be independently interpreted by the transcriptional machinery, possibly through successive enhancer-promoter interactions. These results provide clear evidence that the computational functions usually ascribed to the enhancer itself are actually shared with the basal machinery. In contrast to the autonomous computer model of enhancer function, an information-display or 'billboard' model of enhancer activity may better describe many developmentally regulated transcriptional enhancers.