RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells.
RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells.
复制标题
RNAi 敲低转录因子 Pu.1 在小鼠胚胎干细胞分化中的作用。
DOI：
10.1007/978-1-59745-536-7_10
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Yoder,MervinC
中科院分区：
文献类型：
--
作者：
Zou,Gang-Ming;Thompson,MeredithA;Yoder,MervinC
Murine embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of the preimplantation blastocyst. These cells are primitive and undifferentiated and have the potential to become a wide variety of specialized cell types. Mouse ES cells can be regarded as a versatile biological tool that has led to major advances in our understanding of cell and developmental biology. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used, however, this is a time-consuming and expensive approach. Recently, we have shown that small interfering RNA is an effective strategy to knockdown target gene expression, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This method will be useful to test the function of a wide variety of gene products using the ES cell differentiation system.
