Histological evaluation of a novel phosphorylated pullulan-based pulp capping material: An in vivo study on rat molars
Histological evaluation of a novel phosphorylated pullulan-based pulp capping material: An in vivo study on rat molars
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DOI：
10.1111/iej.13587
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发表时间：
2021-07-07
影响因子：
5
通讯作者：
Sano, Hidehiko
中科院分区：
文献类型：
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作者：
Islam, Rafiqul;Toida, Yu;Sano, Hidehiko
目的评价一种新型三氧化二磷聚合体(MTAPPL)对大鼠直接盖髓后牙髓的影响。方法对56只雄性Wistar大鼠上颌第一磨牙制作96个窝洞。随机分为4组：MTAPPL组、磷酸化普鲁兰(PPL)组、常规MTA组(Nex-Cem MTA、NCMTA；阳性对照)和Super-Bond(SB；阴性对照)组。所有的洞都用SB修复，每隔1天、3天、7天和28天用组织学评分系统观察牙髓反应。统计分析采用Kruskal-Wallis和Mann-Whitney U检验，采用Bonferroni‘s校正，显著性水平设为0.05。DMP1和CD34抗原分别用于成牙本质细胞分化和牙髓血管形成。结果术后第1天，MTAPPL组和NCMTA组均可见轻度炎症细胞，PPL组炎症细胞较少，SB与轻、中度炎症反应有关。PPL组与SB组比较差异有统计学意义(P<0.05)。未观察到矿化组织沉积。第3天，PPL和SB中有中到重度的炎症细胞，而MTAPPL和NCMTA有轻微的炎症反应。在NCMTA、MTAPPL和Sb中观察到最初的矿化组织沉积。MTAPPL与PPL相比差异有统计学意义(P<0.05)。第7天，所有无或轻度炎症反应的受试组均可见一层薄薄的矿化组织。第28天，MTAPPL未见炎症反应，而NCMTA、PPL和SB有轻度炎症反应。MTAPPL组与SB组差异有统计学意义(P<0.05)。MTAPPL、NCMTA和PPL均形成了完整的矿化组织屏障，但差异无统计学意义(p>；0.05)。Sb显示不完全矿化组织屏障，与NCMTA、MTAPPL和PPL有显著差异(p<；0.05)。所有观察天时各组CD34染色均为阳性。结论MTAPPL具有良好的牙髓反应和矿化组织形成，是一种潜在的直接盖髓材料。
Aim To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. Methods Ninety-six cavities were prepared in the maxillary first molars of 56 male Wistar rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the application of pulp capping materials: MTAPPL; phosphorylated pullulan (PPL); a conventional MTA (Nex-Cem MTA, NCMTA; positive control); and Super-Bond (SB; negative control). All cavities were restored with SB and observed for pulpal responses at 1-, 3-, 7- and 28-day intervals using a histological scoring system. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-test with Bonferroni's correction, and the level of significance was set at 0.05. DMP1 and CD34 antigen were used to evaluate odontoblast differentiation and pulpal vascularization, respectively. Results On day 1, mild inflammatory cells were present in MTAPPL and NCMTA groups; fewer inflammatory cells were present in the PPL, whereas SB was associated with a mild-to-moderate inflammatory response. A significant difference was observed between PPL and SB (p < .05). No mineralized tissue deposition was observed. On day 3, moderate-to-severe inflammatory cells were present in PPL and SB, whereas MTAPPL and NCMTA had a mild inflammatory response. Initial mineralized tissue deposition was observed in the NCMTA, MTAPPL and SB. A significant difference was observed between MTAPPL and PPL (p < .05). On day 7, a thin layer of mineralized tissue was observed in all tested groups with no or mild inflammatory response. On day 28, no inflammatory response was observed in MTAPPL, whereas NCMTA, PPL and SB had mild inflammatory responses. A significant difference was observed between MTAPPL and SB (p < .05). Complete mineralized tissue barrier formation was observed in MTAPPL, NCMTA and PPL with no significant difference (p > .05). SB exhibited incomplete mineralized tissue barriers, significantly different from NCMTA, MTAPPL and PPL (p < .05). The staining with CD34 was positive in all the groups on all observation days. Conclusion The favourable pulpal responses and induction of mineralized tissue formation associated with MTAPPL indicate its potential application as a direct pulp capping material.