Improved methods for the solubilization and assay of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Improved methods for the solubilization and assay of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase.
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肝 3-羟基-3-甲基戊二酰辅酶 A 还原酶的溶解和测定的改进方法。
DOI：
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发表时间：
1979
影响因子：
6.5
通讯作者：
A. Fogelman
中科院分区：
文献类型：
--
作者：
P. Edwards;D. Lemongello;A. Fogelman
描述了一种溶解 HMG-CoA 还原酶的方法，该方法可重复地产生大鼠肝微粒体中测定的约 190% 的活性。当微粒体膜以固定浓度冷冻、解冻、在含有 50% 甘油的缓冲液中匀浆并在 37°C 下孵育 60 分钟时，会发生最佳溶解。已经开发出还原酶的快速分光光度测定法并确定了最佳条件。使用该测定，确定了 HMG-CoA 还原酶的动力学，纯化至每分钟每毫克蛋白质氧化 17,400 nmol NADPH 的比活性。
A method for solubilizing HMG-CoA reductase is described that reproducibly yielded approximately 190% of the activity assayed in rat liver microsomes. Optimal solubilization occurred when microsomal membranes were frozen at a fixed concentration, thawed, homogenized in a buffer containing 50% glycerol, and incubated at 37 degrees C for 60 minutes. A rapid spectrophotometric assay of the reductase has been developed and the optimal conditions defined. Using this assay, the kinetics were determined for HMG-CoA reductase purified to a specific activity of 17,400 nmol NADPH oxidized per minute per mg protein.
“慢”蛋白质最终会快速折叠。
DOI：
10.1073/pnas.1303539110
发表时间：
2013
影响因子：
11.1
作者：
Best,RobertB
通讯作者：
Best,RobertB