The junction-associated protein, zonula occludens-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts
The junction-associated protein, zonula occludens-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts
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DOI：
10.1073/pnas.93.20.10779
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发表时间：
1996-10-01
影响因子：
11.1
通讯作者：
Louvard, D
中科院分区：
文献类型：
--
作者：
Gottardi, CJ;Arpin, M;Louvard, D
连接相关蛋白 zonula occlusionns-1 (ZO-1) 是膜相关鸟苷酸激酶同源物家族的成员，被认为对细胞-细胞接触位点的信号转导很重要。我们提供的证据表明，在某些细胞生长条件下，可以在细胞核中检测到 ZO-1。针对 ZO-1 多肽不同部分的两种不同抗体揭示了亚汇合而非汇合细胞培养物中的核染色。表位标记的 ZO-1 也可以在转染细胞的细胞核中检测到，可以在培养的上皮细胞的损伤部位刺激核积累，并且在肠上皮细胞的组织切片中对 ZO-1 进行免疫过氧化物酶检测，显示核标记仅沿着绒毛的外尖。这些结果表明 ZO-1 的核定位与细胞接触的程度和/或成熟度成反比，因为细胞与细胞接触是所涉及的信号通路的专门位点在生长和分化过程中，我们认为 ZO-1 的核积累可能与其在膜相关鸟苷酸激酶同源物信号转导中的作用有关。
The junction-associated protein zonula occludens-1 (ZO-1) is a member of a family of membrane-associated guanylate kinase homologues thought to be important in signal transduction at sites of cell-cell contact, We present evidence that under certain conditions of cell growth, ZO-1 can be detected in the nucleus, Two different antibodies against distinct portions of the ZO-1 polypeptide reveal nuclear staining in subconfluent, but not confluent, cell cultures, An exogenously expressed, epitope-tagged ZO-1 can also be detected in the nuclei of transfected cells, Nuclear accumulation can be stimulated at sites of wounding in cultured epithelial cells, and immunoperoxidase detection of ZO-1 in tissue sections of intestinal epithelial cells reveals nuclear labeling only along the outer tip of the villus, These results suggest that the nuclear localization of ZO-1 is inversely related to the extent and/or maturity of cell contact, Since cell-cell contacts are specialized sites for signaling pathways implicated in growth and differentiation, we suggest that the nuclear accumulation of ZO-1 may be relevant for its suggested role in membrane-associated guanylate kinase homologue signal transduction.