Nanofiber-microsphere (nano-micro) matrices for bone regenerative engineering: a convergence approach toward matrix design.
Nanofiber-microsphere (nano-micro) matrices for bone regenerative engineering: a convergence approach toward matrix design.
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DOI：
10.1093/rb/rbu002
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发表时间：
2014-11
影响因子：
6.7
通讯作者：
Laurencin CT
中科院分区：
文献类型：
--
作者：
Nelson C;Khan Y;Laurencin CT
骨骼是健康和生活质量的重要器官。由于目前骨组织工程治疗的不足，科学家们一直在寻求合成材料作为骨移植替代品的应用。作为具有显著细胞外基质（ECM）的复合有机/无机材料，改善骨移植替代物的一种方式可以是设计受天然ECM网络的物理外观影响的合成基质。在这项工作中，作者评估复合材料，混合支架骨组织工程的基础上，复合陶瓷/聚合物微球支架与合成ECM模拟网络在其孔隙空间。使用热诱导相分离，纳米纤维沉积在结构上健全的微球为基础的支架的孔隙空间与密度成比例的初始聚合物浓度。孔隙率测定和机械测试表明，纤维沉积过程不会导致整体孔隙特征或机械完整性发生显著变化。这些支架在人骨小梁的尺度上显示出足够的机械完整性，并支持培养的小鼠颅骨成骨细胞的粘附和增殖。从自然线索，这些支架可能代表了一个新的途径，先进的骨组织工程支架。
Bone is an essential organ for health and quality of life. Due to current shortfalls in therapy for bone tissue engineering, scientists have sought the application of synthetic materials as bone graft substitutes. As a composite organic/inorganic material with significant extra cellular matrix (ECM), one way to improve bone graft substitutes may be to engineer a synthetic matrix that is influenced by the physical appearance of natural ECM networks. In this work, the authors evaluate composite, hybrid scaffolds for bone tissue engineering based on composite ceramic/polymer microsphere scaffolds with synthetic ECM-mimetic networks in their pore spaces. Using thermally induced phase separation, nanoscale fibers were deposited in the pore spaces of structurally sound microsphere-based scaffold with a density proportionate to the initial polymer concentration. Porosimetry and mechanical testing indicated no significant changes in overall pore characteristics or mechanical integrity as a result of the fiber deposition process. These scaffolds displayed adequate mechanical integrity on the scale of human trabecular bone and supported the adhesion and proliferation of cultured mouse calvarial osteoblasts. Drawing from natural cues, these scaffolds may represent a new avenue forward for advanced bone tissue engineering scaffolds.