The Barley Genome
The Barley Genome
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DOI：
10.1007/978-3-319-92528-8
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
N. Stein;G. Muehlbauer
中科院分区：
文献类型：
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作者：
N. Stein;G. Muehlbauer
本章综述了大麦蛋白质组的研究现状。大麦作为谷物模型和主要作物的重要性反映在大量使用蛋白质组学作为解决基础或应用研究问题的方法的出版物上。通过质谱技术发展的进步，蛋白质组学中的中心分析技术，形成了第一节中概述的用于蛋白质或肽分离和蛋白质鉴定的背景方法。谷物对于大麦作为作物的使用具有重要意义，种子生物学是植物科学的中心主题。因此，大量的研究集中在谷物蛋白质组以及蛋白质组组成在谷物成熟和萌发过程中的变化。单独的章节涵盖非生物和生物胁迫防御反应的研究。下一部分是致力于亚细胞蛋白质组学，细胞器或亚细胞组分的分离是一个强大的战略，以科普复杂的植物蛋白质组。目前典型的分析工具只能覆盖完整蛋白质组的一小部分。关于基因的数量，任何蛋白质组的复杂性都因大量的翻译后修饰和许多潜在的剪接变体而增加。此外，单个蛋白质丰度的动态范围覆盖了许多数量级，超过了当前检测方法的极限。尽管蛋白质组的这种复杂性要求深入的蛋白质分析，但关于例如翻译后修饰的信息不能从其他方法如转录组学中获得。这些方面和潜在的发展是在我们的贡献的最后一节。
The present chapter summarizes the current status of proteome research on barley. The importance of barley as a model for cereals and as a major crop is reflected by a large number of publications using proteomics as an approach to address fundamental or applied research questions. Progress through technological developments in mass spectrometry, the central analytical technique in proteomics, forms the background methodology applied for protein or peptide separation and protein identification as outlined in the first section. The grain is of central relevance for the use of barley as a crop and seed biology is a central topic in plant science. Hence, a large number of studies focus on the grain proteome as well as the changes in proteome composition during grain maturation and germination. Separate sections cover research on abiotic and biotic stress defence responses. The next section is dedicated to subcellular proteomics, isolation of organelles or subcellular fractions being a powerful strategy to cope with the complexity of the plant proteome. Typical current analytical tools can cover only a small fraction of the complete proteome. With regard to the number of genes, any proteome is increased in complexity by a high number of post-translational modifications and many potential splicing variants. In addition, the dynamic range of the individual protein abundance covers many orders of magnitude exceeding the limits of current detection methods. Although this complexity of the proteome is demanding for in-depth protein analysis, information on, e.g. post-translational modifications cannot be derived from other approaches such as transcriptomics. These aspects and potential developments are addressed in the final section of our contribution.