IDENTIFICATION OF BULGARIAN STRAIN 258 OF ENTEROVIRUS-71
IDENTIFICATION OF BULGARIAN STRAIN 258 OF ENTEROVIRUS-71
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DOI：
10.1159/000149088
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发表时间：
1979-01-01
期刊：
影响因子：
4.6
通讯作者：
VOROSHILOVA, MK
中科院分区：
文献类型：
--
作者：
MELNICK, JL;SCHMIDT, NJ;VOROSHILOVA, MK
1975年，保加利亚发生了严重的中枢神经系统疾病流行。在临床、组织病理学和流行病学上，这些病例类似于脊髓灰质炎、无菌性脑膜炎、脑膜脑炎，在某些病例中，类似于脑心肌炎。700例报告病例中约21%发生瘫痪，44例发生致死性结局[参考文献1]。在65例病例中，分离出92株相同血清型的肠道病毒：38株来自CNS，10株来自肠系膜淋巴结和扁桃体，44株来自粪便[参考文献1和2]。从一名3个月大婴儿的脊髓中分离出一种典型的代表性菌株258号，该婴儿在发病第5天死亡，有局灶性脊髓灰质炎的体征，该菌株被选择用于深入研究。交叉中和试验确定了保加利亚258株（Stanchev）与71型肠道病毒美国株（BrCr和JaFr株）和同一类型的瑞典株（Nos. 52343、52500和6041）。从其生物学[参考文献1，2]、物理化学[参考文献3]和抗原特性来看，这次大规模流行病的病原体可归类为肠道病毒71型的成员，这是最近发现的最高致病性肠道病毒之一。
In 1975 in Bulgaria a severe epidemic of central nervous system (CNS) disease occurred. Clinically, histopathologically, and epidemiologically the cases resembled poliomyelitis, aseptic meningitis, meningoencephalitis, and, in some cases, encephalomyocarditis. About 21% of the 700 reported cases developed paralysis, 44 with fatal outcome [ref. 1]. In 65 cases, 92 strains of enterovirus of the same serologic type were isolated: 38 strains from the CNS, 10 from mesenteric lymph nodes and tonsils, and 44 from feces [ref. 1 2]. A typical representative strain, No. 258, isolated from the spinal cord of a 3-month-old baby who died on the 5th day of disease with signs of focal polioencephalitis, was selected for intensive study. Cross-neutralization tests established the antigenic identity of the Bulgarian 258 strain (Stanchev) with American strains of enterovirus type 71 (BrCr and JaFr strains) and Swedish strains of the same type (Nos. 52343, 52500, and 6041). From its biological [ref. 1,2], physicochemical [ref. 3], and antigenic properties, the etiological agent of this large epidemic can be classified as a member of enterovirus type 71, one of the most highly pathogenic of the recently recognized entero-viruses.