Identification and characterization of the major mitochondrial Fe transporter in rice
Identification and characterization of the major mitochondrial Fe transporter in rice
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DOI：
10.4161/psb.6.10.17132
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Nishizawa, Naoko K.
中科院分区：
文献类型：
--
作者：
Bashir, Khurram;Ishimaru, Yasuhiro;Nishizawa, Naoko K.
铁的吸收、转运和区室化对植物细胞功能和生命周期至关重要。尽管我们对植物中Fe稳态的理解取得了快速进展，但直到最近，对Fe从细胞质到线粒体的运输仍知之甚少。对3，993个缺铁突变株系的筛选导致了水稻中主要线粒体铁转运蛋白（MIT）的鉴定和表征。MIT被发现定位于线粒体，并补充线粒体铁转运缺陷的酵母菌株的生长。MIT的敲除导致致死表型，并且在敲除的植物中，几个农艺性状受到损害，例如株高、平均分蘖数、开花天数、生育力和产量。参与铁转运的基因表达的变化表明细胞铁转运的干扰。此外，线粒体铁浓度和线粒体Fe-S酶乌头酸酶的活性显着降低与野生型植物相比。MIT的鉴定是植物铁营养领域的一个重大进展，并将有助于从其他植物物种中克隆旁系同源物。
The uptake, translocation and compartmentalization of Fe are essential for plant cell function and life cycle. Despite rapid progress in our understanding of Fe homeostasis in plants, Fe transport from the cytoplasm to mitochondria was, until recently, poorly understood. The screening of 3,993 mutant lines for symptoms of Fe deficiency resulted in the identification and characterization of a major mitochondrial Fe transporter (MIT) in rice. MIT was found to localize to mitochondria and to complement the growth of a yeast strain defective in mitochondrial Fe transport. The knockout of MIT resulted in a lethal phenotype, and in knock-down plants, several agronomic characteristics were compromised, such as plant height, average number of tillers, days to flower, fertility and yield. Changes in the expression of genes involved in Fe transport suggested a disturbance of cellular Fe transport. Furthermore, the mitochondrial Fe concentration and the activity of the mitochondrial Fe-S enzyme aconitase were significantly reduced compared with wild-type plants. The identification of MIT is a significant advance in the field of plant Fe nutrition and should facilitate the cloning of paralogs from other plant species.