The impact of hypoxia and low glucose on the release of acetylcholine and ATP from the incubated cat carotid body
The impact of hypoxia and low glucose on the release of acetylcholine and ATP from the incubated cat carotid body
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DOI：
10.1016/j.brainres.2009.02.078
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发表时间：
2009-05-13
期刊：
影响因子：
2.9
通讯作者：
Kostuk, Eric
中科院分区：
文献类型：
--
作者：
Fitzgerald, Robert S.;Shirahata, Machiko;Kostuk, Eric
颈动脉体（CB）是一个多模态的传感器，它增加了它的神经输出到孤束核与随后激活的几个反射心肺反应。目前的研究表明，乙酰胆碱（ACh）和三磷酸腺苷（ATP）是猫和大鼠脑束中两种重要的兴奋性神经递质。本研究探讨了缺氧、低糖以及两者共同作用对两种培养的猫脑细胞ACh和ATP释放的影响。根据机构动物护理和使用委员会的政策和法规，采用标准程序制备CB。当正常化到他们的控制，一个显着增加的乙酰胆碱在孵育介质中测量响应缺氧，低葡萄糖，和组合的刺激。当标准化到他们的控制，显着增加ATP在孵育介质中测量响应缺氧和组合的刺激。低血糖组ATP含量升高，但无统计学意义（P>0.05）。其次，将激发阶段的初始3-4或2-3 min时间段标准化为对照阶段的最终3-4或2 -3 min时间段，对于ACh和ATP两者，产生响应于缺氧、低葡萄糖（仅ACh）和组合刺激的显著增加。这些数据表明，在猫中，CB神经输出增加，以响应低葡萄糖的可能性可能主要是由于乙酰胆碱。(C)2009 Elsevier B. V.保留所有权利。
The carotid body (CB) is a polymodal sensor which increases its neural output to the nucleus tractus solitarii with a subsequent activation of several reflex cardiopulmonary responses. Current reports identify acetylcholine (ACh) and adenosine triphosphate (ATP) as two essential excitatory neurotransmitters in the cat and rat CBs. This study explored the impact of hypoxia, low glucose, and the two together on the release of both ACh and ATP from two incubated cat CBs. The CBs were prepared with standard procedures in accordance with the policies and regulations of the institutional Animal Care and Use Committee. When normalized to their controls, a significant increase of ACh in the incubation medium was measured in response to hypoxia, low glucose, and the combined stimuli. When normalized to their controls, a significant increase in ATP in the incubation medium was measured in response to hypoxia and to the combined stimuli. Low glucose generated an increase in ATP which was not statistically significant (P>0.05). Second, normalizing the initial 3-4 or 2-3 min Time Segment of the challenge Stage to the final 3-4 or 2-3 min Time Segment of the control Stage for both ACh and ATP generated significant increases in response to hypoxia, low glucose (ACh only), and the combined stimuli. The data suggested the possibility that in the cat the increased CB neural output in response to low glucose might be due primarily to ACh. (C) 2009 Elsevier B.V. All rights reserved.