IMMUNOGLOBULIN GENE REARRANGEMENT IN IMMATURE B-CELLS
IMMUNOGLOBULIN GENE REARRANGEMENT IN IMMATURE B-CELLS
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DOI：
10.1126/science.6774416
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发表时间：
1980-01-01
期刊：
影响因子：
56.9
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
--
作者：
MAKI, R;KEARNEY, J;TONEGAWA, S
对两种细胞质均有μ链而无轻链的未成熟B细胞，即胎儿肝杂杂瘤细胞和白血病细胞系70Z/3，进行了轻链和重链免疫球蛋白基因的DNA重排检测。在由胎儿肝细胞和肿瘤细胞构建的胎儿肝杂交瘤中，未观察到轻链基因重排，而在70Z/3细胞系中可能发生了κ轻链重排。结果表明，尽管缺乏轻链合成可能是由于缺乏基因重排，但也可能存在转录调控，这可能对未成熟B细胞中轻链免疫球蛋白的表达也很重要。
Two types of immature B cells, namely fetal liver hybridomas and the leukemic cell line 70Z/3, both of which have cytoplasmic μ chains but no light chains, were examined for DNA rearrangements of their light chain and heavy chain immunoglobulin genes. In the fetal liver hybridomas, which were constructed from fetal liver cells and a tumor cell, no light chain gene rearrangement was observed, whereas in the 70Z/3 cell line a κ light chain rearrangement probably occurred. The results suggest that, although the lack of light chain synthesis can be due to a lack of gene rearrangement, there may also be transcriptional regulation, which may also be important for the expression of light chain immunoglobulins in immature B cells.