Evidence for participation of neutrophil gelatinase-associated lipocalin/matrix metalloproteinase-9 (NGAL•MMP-9) complex in the inflammatory response to infection in pregnancies complicated by preterm birth.
Evidence for participation of neutrophil gelatinase-associated lipocalin/matrix metalloproteinase-9 (NGAL•MMP-9) complex in the inflammatory response to infection in pregnancies complicated by preterm birth.
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DOI：
10.1111/aji.12523
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Buhimschi CS
中科院分区：
文献类型：
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作者：
Rood KM;Buhimschi IA;Rodewald Millen K;Bahtiyar MO;Thung S;Summerfield T;Zhao G;Ackerman W 4th;Shellhaas C;Samuels P;Buhimschi CS
中性粒细胞明胶酶相关脂质运载蛋白 (NGAL) 在中性粒细胞中表达，并通过螯合铁参与先天免疫。 NGAL 与基质金属蛋白酶 9 (MMP-9) 复合并延长其明胶分解活性的能力促使我们研究其在妊娠并发早产 (PTB) 和羊膜内感染/炎症 (IAI) 中的作用。我们检测了 308 名接受过羊膜穿刺术且妊娠结局或 PTB 正常的女性的羊水 (AF) 水平。采用qRT-PCR测定胎盘绒毛滋养层和羊膜绒毛膜NGAL mRNA的表达。免疫组织化学用于细胞定位。 AF NGAL 水平受孕龄调节。患有 IAI 和 PTB 的女性的 NGAL、MMP-9 和 NGAL·MMP-9 复合物水平显着升高。羊膜绒毛膜是 NGAL 的来源，与其他炎症条件类似，这种蛋白质可能会增强 MMP-9 的胶原蛋白溶解作用，并在 IAI 并发的妊娠中调节宿主-微生物相互作用。
Neutrophil gelatinase-associated lipocalin (NGAL) is expressed in neutrophils and involved in innate immunity by sequestering iron. NGAL's ability to complex with matrix metalloproteinase-9 (MMP-9) and extend its gelatinolytic activity led us to investigate its role in pregnancies complicated by preterm birth (PTB) and intra-amniotic infection/inflammation (IAI). We assayed the amniotic fluid (AF) levels of NGAL and MMP-9 in 308 women that had a clinically indicated amniocentesis and a normal pregnancy outcome or PTB. qRT-PCR was employed to determine NGAL mRNA expression of placental villous trophoblast and amniochorion. Immunohistochemistry was used for cellular localization. AF NGAL levels were gestational age-regulated. Women with IAI and PTB had significantly higher levels of NGAL, MMP-9 and NGAL•MMP-9 complex. The amniochorion is a source of NGAL and similarly to other inflammatory conditions this protein may augment the collagenolytic effect of MMP-9 and modulate host-microbe interactions in pregnancies complicated by IAI.