Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium
Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium
复制标题
DOI：
10.1007/s00284-016-1161-2
复制
发表时间：
2017-01-01
影响因子：
2.6
通讯作者：
Costa, Cristina
中科院分区：
文献类型：
--
作者：
Curtoni, Antonio;Cipriani, Raffaella;Costa, Cristina
基质辅助激光解吸/电离飞行时间 (MALDI-TOF) 质谱 (MS) 是快速鉴定微生物的有用工具。不幸的是，其直接应用于阳性血培养仍然缺乏标准化程序。在本研究中，我们评估了一种易于快速执行的方案，用于 MALDI-TOF MS 直接鉴定在固体培养基上短期孵育后的阳性血培养物中的微生物。该方案用于评估对 162 份阳性单微生物血培养物中微生物的直接鉴定；在不同的孵育时间（3、5、24 小时），对生长的微生物铜锈进行 MALDI-TOF MS 测定。总体而言，MALDI-TOF MS 在物种水平上与传统方法的一致性在 3、5 和 24 小时分别为 60.5、80.2 和 93.8%。仅考虑细菌，物种水平的识别性能在 3、5 和 24 小时分别为 64.1、85.0 和 94.1%。该协议应用于市售的 MS 分型系统，可能代表一种快速而强大的诊断工具，用于直接识别病原体，并在选定的病例中进行迅速的病原体驱动的抗菌治疗。
Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a useful tool for rapid identification of microorganisms. Unfortunately, its direct application to positive blood culture is still lacking standardized procedures. In this study, we evaluated an easy- and rapid-to-perform protocol for MALDI-TOF MS direct identification of microorganisms from positive blood culture after a short-term incubation on solid medium. This protocol was used to evaluate direct identification of microorganisms from 162 positive monomicrobial blood cultures; at different incubation times (3, 5, 24 h), MALDI-TOF MS assay was performed from the growing microorganism patina. Overall, MALDI-TOF MS concordance with conventional methods at species level was 60.5, 80.2, and 93.8% at 3, 5, and 24 h, respectively. Considering only bacteria, the identification performances at species level were 64.1, 85.0, and 94.1% at 3, 5, and 24 h, respectively. This protocol applied to a commercially available MS typing system may represent, a fast and powerful diagnostic tool for pathogen direct identification and for a promptly and pathogen-driven antimicrobial therapy in selected cases.