Glucose-triggered co-enzymatic hydrogelation of aqueous polymer solutions
Glucose-triggered co-enzymatic hydrogelation of aqueous polymer solutions
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DOI：
10.1039/c1ra01060c
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发表时间：
2012-01-01
期刊：
影响因子：
3.9
通讯作者：
Taya, Masahito
中科院分区：
文献类型：
--
作者：
Sakai, Shinji;Komatani, Kimiko;Taya, Masahito
利用葡萄糖氧化酶（GOx）催化的葡萄糖氧化反应生成H2 O2，在辣根过氧化物酶（HRP）催化的含酚羟基（PH）聚合物水溶液的水凝胶化反应中提供H2 O2。使用带有PH部分的藻酸盐衍生物（Alg-PH）评估葡萄糖触发的水凝胶化的可行性。初始速率之间的交联形成的PH部分和凝胶化时间的Alg-PH水溶液的增加和减少，分别与GOx和葡萄糖浓度的增加。通过改变这些参数，可以得到具有与使用常规的直接H2 O2添加系统形成的交联密度和凝胶化时间相当的水凝胶。所得水凝胶的机械性能也是可控的。在1 μ mL（-1）HRP、0.8 μ mL（-1）GOx和4.6 mM葡萄糖条件下，从1.1%（w/v）Alg-PH溶液中获得的水凝胶显示出比用1 μ mL（-1）HRP和4.6 mM H_2O_2获得的水凝胶所显示的更大的对压缩的排斥力。这些结果表明，葡萄糖引发的水凝胶化系统具有广泛的生物医学应用的潜力，如制造组织工程的基板，药物和蛋白质递送系统的载体，和伤口敷料。
H2O2 generation by glucose oxidase (GOx)-catalysed oxidation of glucose was used to supply H2O2 in the horseradish peroxidase (HRP)-catalysed hydrogelation of aqueous solutions of polymers bearing phenolic hydroxyl (PH) moieties. The feasibility of the glucose-triggered hydrogelation was evaluated using an alginate derivative bearing PH moieties (Alg-PH). The initial rate of cross-link formation between the PH moieties and the gelation time of the Alg-PH aqueous solution increased and decreased, respectively, with increasing GOx and glucose concentrations. Hydrogels with comparable cross-link densities and gelation times to those formed using a conventional direct H2O2 addition system could be obtained by altering these parameters. The mechanical properties of the resultant hydrogels were also controllable. The hydrogel obtained from a 1.1% (w/v) Alg-PH solution at 1 u mL(-1) HRP, 0.8 u mL(-1) GOx, and 4.6 mM glucose showed repulsion forces toward compression more than four times those shown by hydrogels obtained with 1 u mL(-1) HRP and 4.6 mM H2O2. These results indicate that the glucose-triggered hydrogelation system has potential for a wide range of biomedical applications such as fabricating substrates for tissue engineering, carriers for drug and protein delivery systems, and wound dressings.