MECHANICALLY INDUCED ELECTRICAL AND INTRACELLULAR CALCIUM RESPONSES IN NORMAL AND CANCEROUS MAMMARY CELLS
MECHANICALLY INDUCED ELECTRICAL AND INTRACELLULAR CALCIUM RESPONSES IN NORMAL AND CANCEROUS MAMMARY CELLS
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DOI：
10.1016/0143-4160(92)90018-n
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发表时间：
1992-08-01
期刊：
影响因子：
4
通讯作者：
MAENO, T
中科院分区：
文献类型：
--
作者：
ENOMOTO, K;FURUYA, K;MAENO, T
Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca2+-dependent K+ channel or outward membrane current was recorded in cells which were several cells distant from the stimulated cell. Mechanical stimulation also induced an increase of [Ca2+]i in the touched cell, and this increase of [Ca2+]i spread to the surrounding cells. The [Ca2+]i signal travelled a distance of 100-200-mu-m within 20-40 s and then diminished. The presence of cell-to-cell communication between adjacent mammary cells through gap junction was indicated by injection of lucifer yellow and measurements of electrical coupling (coupling constant 0.2-0.3). The mechanically induced increase of the [Ca2+]i signal spread to adjacent cells even when the stimulated cell had no physical contact with them. In the absence of fluid movement, the pattern of the spread of the [Ca2+]i signal was a concentric circle. However, in the presence of fluid movement, the pattern changed to elongate to the direction of the flow. These findings suggested that a certain factor was released from the mechanically stimulated cell to the extracellular space, and this factor induces the increase of [Ca2+]i in surrounding cells.
