Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)
Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)
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DOI：
10.1016/j.carbpol.2016.03.043
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发表时间：
2016-08-01
影响因子：
11.2
通讯作者：
Park, Yong Il
中科院分区：
文献类型：
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作者：
Choi, Ji Won;Synytsya, Andriy;Park, Yong Il
从韩国桑果 Oddi (Morus alba L.) 中分离得到水溶性多糖 JS-MP-1。糖键分析和NMR数据证实它是带有阿拉伯聚糖和阿拉伯半乳聚糖(AG II)侧链的鼠李糖半乳糖醛酸I型(RG I)聚合物。 JS-MP-1 剂量依赖性地降低 3T3-L1 前脂肪细胞的活力，显着刺激 caspase 9 和 3 以及聚（ADP-核糖）聚合酶（PARP）的裂解，并降低 Bcl-2 与 Bax 表达水平的比率，从而导致前脂肪细胞线粒体功能障碍和凋亡。细胞凋亡是通过刺激 MAPK（ERK 和 p38）信号通路介导的。这些结果表明，JS-MP-1能够通过抑制前脂肪细胞增殖来减少脂肪细胞的数量和脂肪组织的质量，因此JS-MP-1本身或含有这种多糖的粗水性Oddi提取物可以用作有益于健康的食品补充剂的功能成分，用于治疗或预防肥胖症。 (C) 2016 Elsevier Ltd. 保留所有权利。
A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders. (C) 2016 Elsevier Ltd. All rights reserved.