Coexpression of truncated human cytomegalovirus gH with the UL115 gene product or the truncated human fibroblast growth factor receptor results in transport of gH to the cell surface.
Coexpression of truncated human cytomegalovirus gH with the UL115 gene product or the truncated human fibroblast growth factor receptor results in transport of gH to the cell surface.
复制标题
截短的人巨细胞病毒 gH 与 UL115 基因产物或截短的人成纤维细胞生长因子受体共表达，导致 gH 转运至细胞表面。
作者：
Spaete,RR;Perot,K;Scott,PI;Nelson,JA;Stinski,MF;Pachl,C
疱疹病毒的gH糖蛋白在病毒感染的细胞中位于细胞表面，但在重组表达载体上单独表达时保留在内质网（ER）中。这些观察结果表明，需要病毒功能或病毒诱导的细胞功能来促进gH的表面表达，gL在单纯疱疹病毒（HSV）感染的细胞中发挥了这一作用（J. Virol.66, 2240-2250, 1992）。我们已经鉴定出UL115开放阅读框（ORF）的基因产物在人巨细胞病毒（CMV）基因组中是HSV gL的功能同源物。此外，我们已经证明了一个细胞基因，人碱性成纤维细胞生长因子受体（FGFr）也会促进CMV gH向细胞表面的运输。UL115 ORF基因产物或可溶性fgfrc末端截断gH在中国仓鼠卵巢细胞中的共表达可提高gH分泌水平。这些研究表明，当重组gH在病毒感染细胞外表达时，共表达的分子可以掩盖暴露的内质网保留信号。
The gH glycoprotein of herpesviruses is located on the cell surface in viral-infected cells but is retained in the endoplasmic reticulum (ER) when expressed separately from a recombinant expression vector. These observations suggested the requirement for either a viral function or a viral-induced cellular function which facilitates surface expression of gH, gL fulfills this role in the herpes simplex virus (HSV)-infected cell (J. Virol.66, 2240-2250, 1992). We have identified the gene product of the UL115 open reading frame (ORF) as the functional homologue of HSV gL in the human cytomegalovirus (CMV) genome. In addition, we have demonstrated that a cellular gene, the human basic fibroblast growth factor receptor (FGFr) will also facilitate some transport of CMV gH to the cell surface. Coexpression in Chinese hamster ovary cells of the gene product of the UL115 ORF or soluble FGFrwith C-terminally truncated gH enhanced levels of secreted gH. These studies suggest that the coexpressed molecules act to mask an ER retention signal(s) exposed when recombinant gH is expressed outside of the context of the viral-infected cell.