High quality de novo genome assembly of non-conventional yeast Kazachstania bulderi a new potential low pH production host for biorefineries
High quality de novo genome assembly of non-conventional yeast Kazachstania bulderi a new potential low pH production host for biorefineries
复制标题
DOI：
10.1101/2023.01.11.523663
复制
发表时间：
2023-01
期刊：
影响因子：
--
通讯作者：
L. N. Balarezo-Cisneros;S. Timouma;A. Hanak;A. Currin;F. Valle;D. Delneri
中科院分区：
文献类型：
--
作者：
L. N. Balarezo-Cisneros;S. Timouma;A. Hanak;A. Currin;F. Valle;D. Delneri
Kazachstania bulderi是一种普遍存在的非常规酵母，具有在低pH下对葡萄糖和δ-葡萄糖酸内酯有效生长的能力。这种独特的特性使k.b ulderi成为低pH发酵过程中可持续生产有机酸等绿色化学品的理想新宿主。为了加速该物种的品系发育，需要详细的遗传信息。在这里，通过采用高精度长读测序，我们报告了属于K. bulderi种的三个菌株，包括型菌株的高质量阶段基因组组装。这些序列被组装成12条染色体，总长度为14Mb，并在结构和功能水平上对基因组进行了完整的注释，包括等位基因和结构变异、核糖体阵列、着丝粒和交配型位点。这个高质量的参考基因组提供了一个重要的资源，以推进我们对生物技术相关的非常规酵母的基础知识，并支持开发遗传工具来操纵这些菌株，使其成为生物技术生产过程的宿主。
Kazachstania bulderi is a yeast species belonging to a ubiquitous group of non-conventional yeasts which has the ability to grow efficiently on glucose and δ-gluconolactone at low pH. This unique trait makes K. bulderi an ideal candidate as a new host for low pH fermentation processes for sustainable production of green chemicals such as organic acids. To accelerate strain development with this species, detailed information of its genetics is needed. Here, by employing high accuracy long read sequencing we report a high-quality phased genome assembly for three strains belonging to K. bulderi species, including the type strain. The sequences were assembled into 12 chromosomes with a total length of 14Mb, and the genome was fully annotated at structural and functional levels, including allelic and structural variants, ribosomal array, centromeres and mating type locus. This high-quality reference genome provides an essential resource to advance our fundamental knowledge of biotechno-logically relevant non-conventional yeasts and to support the development of genetic tools for manipulating such strains towards their use as production hosts biotechnological processes.