BnaC9.SMG7b Functions as a Positive Regulator of the Number of Seeds per Silique in Brassica napus by Regulating the Formation of Functional Female Gametophytes
BnaC9.SMG7b Functions as a Positive Regulator of the Number of Seeds per Silique in Brassica napus by Regulating the Formation of Functional Female Gametophytes
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BnaC9.SMG7b 通过调节功能性雌配子体的形成，作为甘蓝型油菜每角果种子数的正调节因子
DOI：
10.1104/pp.15.01040
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发表时间：
2015-12-01
期刊：
影响因子：
7.4
通讯作者：
Yang, Guangsheng
中科院分区：
文献类型：
--
作者：
Li, Shipeng;Chen, Lei;Yang, Guangsheng
每角果粒数（NSS）是油菜科作物种子产量潜力的重要决定因素，它受自然发生的数量性状基因座控制。我们先前定位了一个控制甘蓝型油菜NSS的C9染色体上的主要数量性状位点qSS.C9。更好地了解qSS.C9如何控制B中的NSS。napus中，我们通过基于图位的克隆策略分离了该位点。qSS.C9编码一个119个氨基酸的小蛋白，命名为BnaC9.SMG7b，与拟南芥端粒1四肽重复序列和端粒中心结构域同源，对生殖器7有形态发生作用。BnaC9.SMG7b在功能性雌配子体的形成中起调节作用，从而决定功能性大孢子的形成，进而决定成熟胚珠的形成。BnaC9.SMG7b的自然损失或人工敲低显著减少了每个角果的功能胚珠的数量，因此导致种子数量减少，表明qSS。C9是B中NSS的正调节因子。油菜。序列和功能分析表明，BnaC9.SMG7b经历了一个亚功能化过程，导致无义介导的mRNA衰变中的功能丧失，例如在拟南芥SMG7中。单倍型分析表明，BnaC9. SMG 7 B b等位基因在现代B中普遍存在。这表明该位点是B的主要选择目标。油菜改良我们的研究结果代表了解开控制B中NSS自然变异的分子机制的第一步。油菜。
Number of seeds per silique (NSS) is an important determinant of seed yield potential in Brassicaceae crops, and it is controlled by naturally occurring quantitative trait loci. We previously mapped a major quantitative trait locus, qSS.C9, on the C9 chromosome that controls NSS in Brassica napus. To gain a better understanding of how qSS.C9 controls NSS in B. napus, we isolated this locus through a map-based cloning strategy. qSS.C9 encodes a predicted small protein with 119 amino acids, designated as BnaC9.SMG7b, that shows homology with the Ever ShorterTelomere1 tertratricopeptide repeats and Ever Shorter Telomere central domains of Arabidopsis (Arabidopsis thaliana) SUPPRESSOR WITH MORPHOGENETIC EFFECTS ON GENITALIA7 (SMG7). BnaC9.SMG7b plays a role in regulating the formation of functional female gametophyte, thus determining the formation of functional megaspores and then mature ovules. Natural loss or artificial knockdown of BnaC9.SMG7b significantly reduces the number of functional ovules per silique and thus, results in decreased seed number, indicating that qSS. C9 is a positive regulator of NSS in B. napus. Sequence and function analyses show that BnaC9.SMG7b experiences a subfunctionalization process that causes loss of function in nonsense-mediated mRNA decay, such as in Arabidopsis SMG7. Haplotype analysis in 84 accessions showed that the favorable BnaC9.SMG7b alleles are prevalent in modern B. napus germplasms, suggesting that this locus has been a major selection target of B. napus improvement. Our results represent the first step toward unraveling the molecular mechanism that controls the natural variation of NSS in B. napus.