Intrahepatic detection of FOXP3 gene expression after liver transplantation using minimally invasive aspiration biopsy
Intrahepatic detection of FOXP3 gene expression after liver transplantation using minimally invasive aspiration biopsy
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DOI：
10.1097/01.tp.0000258597.97468.88
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发表时间：
2007-03-27
期刊：
影响因子：
6.2
通讯作者：
van der Laan, Luc J. W.
中科院分区：
文献类型：
--
作者：
Demirkiran, Ahmet;Baan, Carla C.;van der Laan, Luc J. W.
Forkhead box P3（FOXP 3）阳性调节性T细胞（Treg）的移植物内蓄积与移植模型中同种异体反应的局部抑制相关。在本研究中，微创细针穿刺活检的实用性的移植物内检测FOXP 3和干扰素（IFN）-γ mRNA的表达进行了研究，在临床肝移植（LTx）。移植物内FOXP 3在LTx后的第一年内增加，但血液中没有。在丙型肝炎病毒（HCV）再感染和先前急性排斥反应发作后，观察到肝脏中FOXP 3表达升高，但IFN-γ表达未升高。这些数据显示了抽吸活检用于移植物内基因表达监测的可行性。肝内FOXP 3水平与HCV再感染、急性排斥史相关，并且在LTx后第一年内升高。移植物和血液之间基因表达的差异强调了局部免疫监测的重要性。
Intragraft accumulation of Forkhead box P3 (FOXP3)-positive regulatory T cells (Treg) is associated with local suppression of alloresponses in transplantation models. In the current study, the utility of the minimally invasive fine needle aspiration biopsy for the intragraft detection of FOXP3 and interferon (IFN)-gamma mRNA expression was investigated in clinical liver transplantation (LTx). Intragraft FOXP3 increased within the first year after LTx, but not in blood. Elevated FOXP3, but not IFN-gamma expression, in the liver was observed after hepatitis C virus (HCV) reinfection and after a previous episode of acute rejection. These data show the feasibility of aspiration biopsy for intragraft monitoring of gene expression. Intrahepatic FOXP3 levels are associated with HCV reinfection, a history of acute rejection, and increased within the first year after LTx. Differences in gene expression between the graft and blood underline the importance of local immune monitoring.