Identification of a Hyperpolarization-activated Cyclic Nucleotide-gated Channel and Its Subtypes in the Urinary Bladder of the Rat
Identification of a Hyperpolarization-activated Cyclic Nucleotide-gated Channel and Its Subtypes in the Urinary Bladder of the Rat
复制标题
大鼠膀胱中超极化激活的环核苷酸门控通道及其亚型的鉴定
DOI：
10.1016/j.urology.2012.01.037
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发表时间：
2012-06-01
期刊：
影响因子：
2.1
通讯作者：
Li, Longkun
中科院分区：
文献类型：
--
作者：
He, Peng;Deng, Jianping;Li, Longkun
目的探讨超极化激活环核苷酸门控（HCN）通道及其亚型在膀胱组织，尤其是膀胱Cajal间质细胞（ICC）中的分布和作用。双标记荧光法检测到膀胱ICC中存在HCN 1亚型。结果用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blotting）检测到HCN 1 -4亚型在膀胱ICC中的表达。基于我们的半定量分析，发现HCN 1是最突出的同种型。通过HCN 1与试剂盒（CD 117）的联合标记，用双标记荧光法证实HCN 1在膀胱ICCs中的表达。结论膀胱内存在4种HCN通道亚型，它们可能通过膀胱ICC影响膀胱兴奋。泌尿学79：1411.e7-1411.e13，2012。皇冠版权所有（C）2012由Elsevier Inc.发布。
OBJECTIVE To investigate the distribution and effects of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel and its isoforms in bladder, especially in bladder interstitial cells of Cajal (ICC).METHODS Four HCN isoforms were detected in bladder tissue from rats using reverse transcription-polymerase chain reaction and Western blotting. The HCN1 subtype was observed in bladder ICCs by double-labeled fluorescence. The effect of the HCN blocker, ZD7288, was investigated using the bladder smooth muscle strip test.RESULTS HCN1-4 isoforms were all identified in bladder ICCs using reverse transcription-polymerase chain reaction and Western blotting. Based on our semiquantitative analysis, HCN1 was found to be the most prominent isoform. The expression of HCN1 was confirmed in bladder ICCs by double-labeled fluorescence through colabeling of HCN1 and kit (CD117). ZD7288 significantly decreased the bladder excitation.CONCLUSION All 4 HCN channel isoforms exist in the bladder, and they affect the bladder excitation, presumably via bladder ICCs. UROLOGY 79: 1411.e7-1411.e13, 2012. Crown Copyright (C) 2012 Published by Elsevier Inc.