The role of the nonciliated bronchiolar epithelial (Clara) cell as the progenitor cell during bronchiolar epithelial differentiation in the perinatal rabbit lung.
The role of the nonciliated bronchiolar epithelial (Clara) cell as the progenitor cell during bronchiolar epithelial differentiation in the perinatal rabbit lung.
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DOI：
10.1165/ajrcmb/7.6.606
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发表时间：
1992-12
影响因子：
6.4
通讯作者：
C. Plopper;S. Nishio;J. L. Alley;P. Kass;D. Hyde
中科院分区：
文献类型：
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作者：
C. Plopper;S. Nishio;J. L. Alley;P. Kass;D. Hyde
尽管已经明确无纤毛细支气管上皮 (Clara) 细胞在细支气管上皮损伤后可作为其自身和成体肺中纤毛细胞的祖细胞，但发育过程中这种关系的性质尚未得到很好的表征。为了确定细支气管纤毛细胞和非纤毛细胞的增殖和分化模式，将新西兰白兔的胎儿和后代的肺（年龄范围从妊娠 24 天到产后 25 周（PN））通过气道输注固定并包埋以进行同步光和透射电子显微镜检查。终末细支气管中可以区分出三类细胞：具有丰富糖原和不同数量细胞器的无纤毛细胞；无纤毛细胞，糖原很少，多聚核糖体数量较多，基体数量不等；和具有不同高度纤毛的纤毛细胞。在 24 天和 27 天孕龄 (DGA) 时，两种类型的非纤毛细胞合计占上皮细胞的 100%。在 DGA 30 天时，他们占人口的 85%；在所有出生后年龄，他们占总人口的 75% 至 81%。在 24 DGA 和 1 周 PN 之间，具有多核糖体和基体的非纤毛细胞占总非纤毛细胞群的 10% 至 20%，此后未发现。在年龄小于 30 DGA 的动物中未观察到纤毛细胞。放射自显影测定妊娠兔注射氚化胸苷后胎儿细支气管上皮的标记指数，28 DGA（注射后 1 小时 [PI]）时为 57 个细胞/千个，29 DGA（24 小时 PI）时为 76 个细胞/千个，30 DGA（48 小时 PI）时为 114 个细胞。（摘要截断为 250 字）
Although it is well established that the nonciliated bronchiolar epithelial (Clara) cell serves as the progenitor for itself and ciliated cells in the adult lung following bronchiolar epithelial injury, the nature of this relationship during development has not been well characterized. To define the pattern of proliferation and differentiation of bronchiolar ciliated and nonciliated cells, lungs of fetuses and offspring from time-mated New Zealand White rabbits, ranging in age from 24 days of gestation to 25 wk postnatal (PN), were fixed by airway infusion and embedded for simultaneous light and transmission electron microscopy. Three categories of cells could be distinguished in terminal bronchioles: nonciliated cells with abundant glycogen and variable numbers of organelles; nonciliated cells with little glycogen, large numbers of polyribosomes, and variable numbers of basal bodies; and ciliated cells with cilia of varying height. Together, both types of nonciliated cells were 100% of the epithelium at 24 and 27 days gestation age (DGA). At 30 days DGA, they were 85% of the population; at all postnatal ages, they ranged from 75 to 81% of the total population. Nonciliated cells with polyribosomes and basal bodies were 10 to 20% of the total nonciliated cell population between 24 DGA and 1 wk PN and not found thereafter. Ciliated cells were not observed in animals younger than 30 DGA. Labeling indices of bronchiolar epithelium in fetuses of pregnant rabbits injected with tritiated thymidine, as determined by autoradiography, were 57 cells per thousand at 28 DGA (1 h postinjection [PI]), 76 at 29 DGA (24 h PI), and 114 at 30 DGA (48 h PI).(ABSTRACT TRUNCATED AT 250 WORDS)