Bacterial transcytosis across conjunctival M cells.
Bacterial transcytosis across conjunctival M cells.
复制标题
结膜 M 细胞的细菌转胞吞作用。
DOI：
10.1167/iovs.06-1202
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发表时间：
2007
影响因子：
4.4
通讯作者：
Phillips,ThomasE
中科院分区：
文献类型：
--
作者：
Petris,CarisaK;Golomb,Miriam;Phillips,ThomasE
目的.已在兔和豚鼠淋巴滤泡上的结膜组织中鉴定出抗原采样M细胞。Maackia amurensis leukoagglutinin（MAL）-I选择性标记显示豚鼠结膜M细胞表面有α（2-3）唾液酸。流感嗜血杆菌菌株OM 12，表达HMW 1粘附α（2-3）唾液酸，和Rd KW 20，缺乏HMW 1，被用来测试假设结膜M细胞易位大微生物。将万古霉素标记的细菌滴入结膜囊中长达130分钟。共聚焦激光扫描显微镜和电子显微镜被用来可视化细菌分布。M细胞，而不是眼睑区域的非滤泡上皮细胞，选择性地结合和移位细菌。到66分钟时，在三维重建中发现卵泡相关上皮（FAE）表面为423±165个细菌/mm 2，延伸至表面以下15.4 μm。到127分钟时，FAE表面的细菌数量增加到579±44个/mm 2，并且它们已经移动到卵泡更深处的50%。与MAL-I共同施用使OM 12转运减少61%。同样，Rd KW 20摄取在63分钟时减少71%，在121分钟时减少58%，表明OM 12摄取至少部分由与α（2-3）唾液酸结合介导。结膜M细胞是大型微生物的进入口，可能在启动针对结膜或短暂性眼部细菌物种的粘膜免疫应答中发挥作用，并可能允许病原体进入。
purpose. Antigen-sampling M cells have been identified in conjunctival tissue overlying lymphoid follicles in rabbits and guinea pigs. Conjunctival M cells in the guinea pig display α (2-3) sialic acid on their surfaces, as evinced by selective labeling by Maackia amurensis leukoagglutinin (MAL)-I. Haemophilus influenzae strains OM12, which expresses the HMW1 adhesin for α (2-3) sialic acid, and Rd KW20, which lacks HMW1, were used to test the hypothesis that conjunctival M cells translocate large microbes.methods. Fluorescein-labeled bacteria were instilled into the conjunctival sac for up to 130 minutes. Confocal laser scanning microscopy and electron microscopy were used to visualize bacterial distribution.results. M cells, but not nonfollicular epithelial cells in the palpebral region, selectively bound and translocated bacteria. By 66 minutes, 423±165 bacteria/mm 2 of follicle-associated epithelial (FAE) surface were found in three-dimensional reconstructions extending 15.4 μm below the surface. By 127 minutes, the number of bacteria increased to 579±44/mm 2 of FAE surface and they had moved 50% deeper into the follicle. Coadministration with MAL-I reduced OM12 transport by 61%. Similarly, Rd KW20 uptake was 71% less at 63 minutes and 58% less at 121 minutes, indicating that OM12 uptake is at least partially mediated by binding to α (2-3) sialic acid.conclusions. Conjunctival M cells are a port of entry for large microbes and may play a role in initiation of mucosal immune responses against commensal or transient ocular bacterial species and may allow the entry of pathogens.