CHROMATOGRAPHIC STUDY OF THE THERMODYNAMIC AND KINETIC CHARACTERISTICS OF SILICA-BOUND CONCANAVALIN-A
CHROMATOGRAPHIC STUDY OF THE THERMODYNAMIC AND KINETIC CHARACTERISTICS OF SILICA-BOUND CONCANAVALIN-A
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DOI：
10.1016/s0021-9673(01)87800-6
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
CARR, PW
中科院分区：
文献类型：
--
作者：
MULLER, AJ;CARR, PW
开发了一种使用高效液体亲和色谱法测定糖衍生物从二氧化硅固定的刀豆球蛋白 A 解离速率常数的方法。观察到的速率常数为.apprx。比溶液中报道的小 10 倍。 10-μm二氧化硅颗粒的使用表明，样品从固定的亲和配体的缓慢解离是导致在该系统中观察到的大板高度的主要因素。评估了几个色谱变量（温度、流动相改性剂和粒径）对观察到的解离速率常数和峰质心的影响。
A method was developed for determining the rate constants for dissociation of sugar derivatives from silica-immobilized concanavalin A using high-performance liquid affinity chromatography. The rate constants observed are .apprx. 10-fold smaller than those reported in solution. The use of 10-.mu.m silica particles showed that slow dissociation of the sample from the immobilized affinity ligand is the predominant factor responsible for the large plate heights observed in this system. The effect of several chromatographic variables (temperature, mobile phase modifiers and particle size) on the observed dissociation rate constant and peak centroid were evaluated.