IDENTIFICATION OF 9 TISSUE-SPECIFIC TRANSCRIPTION FACTORS OF THE HEPATOCYTE NUCLEAR FACTOR-III FORKHEAD DNA-BINDING-DOMAIN FAMILY
IDENTIFICATION OF 9 TISSUE-SPECIFIC TRANSCRIPTION FACTORS OF THE HEPATOCYTE NUCLEAR FACTOR-III FORKHEAD DNA-BINDING-DOMAIN FAMILY
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DOI：
10.1073/pnas.90.9.3948
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发表时间：
1993-05-01
影响因子：
11.1
通讯作者：
COSTA, RH
中科院分区：
文献类型：
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作者：
CLEVIDENCE, DE;OVERDIER, DG;COSTA, RH
肝细胞核因子(HNF)-3 α， -3 β和-3 γ是肝脏转录因子，介导许多肝细胞特异性基因的协调表达，HNF-3蛋白之间以及与果蝇同源蛋白叉头（fkh）具有dna结合域同源性。HNF-3/fkh DNA结合域构成了一个未被识别的蛋白质基元，该基元将其同源DNA结合位点识别为单体。已知在果蝇和非洲爪蟾的胚胎发生过程中需要额外的HNF-3/fkh相关蛋白。在本报告中，我们描述了通过低严格杂交和聚合酶链反应方案从啮齿动物组织cdna中分离出另外9个HNF-3/fkh同源（HFH）克隆。许多HFH基因表现出组织限制性表达模式，并在肝脏以外的组织中转录，包括脑、肾、肺和肠。因此，HNF-3/fkh基序包含一个大的转录因子基因家族，在组织特异性基因调控和发育中发挥作用。
Hepatocyte nuclear factor (HNF)-3alpha, -3beta, and -3gamma are liver transcription factors that mediate the coordinate expression of a number of hepatocyte-specific genes, The HNF-3 proteins share DNA-binding-domain homology among themselves and with the Drosophila homeotic protein forkhead (fkh). The HNF-3/fkh DNA-binding domain constitutes an uncharacterized protein motif that recognizes its cognate DNA binding site as a monomer. Additional HNF-3/fkh-related proteins are known to be required for determination events during embryogenesis in Drosophila and Xenopus. In this report, we describe the isolation of nine additional HNF-3/fkh homologue (HFH) clones from rodent tissue cDNAs by using both low-stringency hybridization and a polymerase chain reaction protocol. Many of the HFH genes exhibit a tissue-restricted expression pattern and are transcribed in tissues other than liver, including brain, kidney, lung, and intestine. The HNF-3/fkh motif therefore comprises a large gene family of transcription factors that play a role in tissue-specific gene regulation and development.