Dendritic Cells and T Cells Interact Within Murine Afferent Lymphatic Capillaries
Dendritic Cells and T Cells Interact Within Murine Afferent Lymphatic Capillaries
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DOI：
10.3389/fimmu.2019.00520
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发表时间：
2019-03-22
影响因子：
7.3
通讯作者：
Halin, Cornelia
中科院分区：
文献类型：
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作者：
Hunter, Morgan Campbell;Teijeira, Alvaro;Halin, Cornelia
传入淋巴管通过将抗原和白细胞转运到引流淋巴结（LN）来促进免疫，并且正在成为外周耐受调节的新参与者。在发炎的鼠耳皮肤中进行活体显微镜检查，我们发现迁移的树突状细胞（DC）和抗原经历的效应T细胞花费相当长的时间在传入毛细淋巴管内停滞或聚集。我们还观察到淋巴内T细胞经常与DC相互作用。当成像多克隆T细胞在一个正在进行的接触超敏反应，大多数淋巴内DC-T细胞的相互作用是短暂的。相反，在迟发型超敏反应期间，同源抗原携带DC参与与抗原特异性T细胞的长寿命MHCII-（I-A/I-E）依赖性相互作用。长寿命的淋巴内DC-T细胞相互作用降低了DC爬行的速度，但并没有延迟整体DC迁移到引流淋巴结。虽然这些淋巴内相互作用的进一步后果仍需要探索，但我们的研究结果表明，毛细淋巴管代表了一个独特的隔室，其中发生了适应性免疫相互作用和调节。
Afferent lymphatic vessels contribute to immunity by transporting antigen and leukocytes to draining lymph nodes (LNs) and are emerging as new players in the regulation of peripheral tolerance. Performing intravital microscopy in inflamed murine ear skin we found that migrating dendritic cells (DCs) and antigen-experienced effector T cells spend considerable time arresting or clustering within afferent lymphatic capillaries. We also observed that intralymphatic T cells frequently interacted with DCs. When imaging polyclonal T cells during an ongoing contact-hypersensitivity response, most intralymphatic DC-T cell interactions were short-lived. Conversely, during a delayed-type-hypersensitivity response, cognate antigen-bearing DCs engaged in long-lived MHCII-(I-A/I-E)-dependent interactions with antigen-specific T cells. Long-lived intralymphatic DC-T cell interactions reduced the speed of DC crawling but did not delay overall DC migration to draining LNs. While further consequences of these intralymphatic interactions still need to be explored, our findings suggest that lymphatic capillaries represent a unique compartment in which adaptive immune interaction and modulation occur.