Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens
Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens
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DOI：
10.1016/j.pestbp.2006.09.004
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发表时间：
2007-05-01
影响因子：
4.7
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
--
作者：
Hu, Xiaobang;Sun, Yan;Zhu, Changliang
从淡色库蚊（Culex pipiens pallens）中克隆了一个视蛋白基因NYD-OP 7。该基因的开放阅读框（ORF）为1116 bp，编码371个氨基酸，与伊蚊和按蚊视蛋白具有高度同源性。NYD-OP 7的转录表达在所有生命阶段的溴氰菊酯敏感和抗性品系的库蚊通过实时PCR测定。结果表明，该基因在所有发育阶段均表达，且主要在蛹和成虫阶段表达。同时，在蛹和成虫中，NYD-OP 7在溴氰菊酯抗性品系中的表达高于敏感品系。重要的是，NYD-OP 7在蚊子C6/36细胞中的稳定表达可赋予中度溴氰菊酯抗性。我们的研究提供了第一个直接的证据表明，增加的视蛋白基因的表达可能发挥一定的作用，在溴氰菊酯抗性的发展在Cx。pipiens pallens (c)2006年爱思唯尔公司All rights reserved.
One mosquito opsin gene, NYD-OP7, has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 1116bp was found to encode a putative 371 amino acids protein which exhibits high identity with opsins from Aedes and Anopheles mosquitoes. Transcript expression of NYD-OP7 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains of the Culex mosquito. The results demonstrated that this gene is expressed at all developmental stages, and it is expressed predominantly at the pupae and adult stages. Meanwhile, in pupae and adults, NYD-OP7 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Importantly, stable expression of NYD-OP7 in the mosquito C6/36 cells can confer moderate deltamethrin resistantce. Our study provides the first direct evidence that increased expression of an opsin gene may play some role in the development of deltamethrin resistantce in Cx. pipiens pallens. (c) 2006 Elsevier Inc. All rights reserved.