Analysis of the genome of leporid herpesvirus 4.
Analysis of the genome of leporid herpesvirus 4.
复制标题
兔疱疹病毒4型基因组分析。
DOI：
10.1016/j.virol.2012.08.002
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jin,Ling
中科院分区：
文献类型：
--
作者：
Babra,Bobby;Watson,Gregory;Xu,Wayne;Jeffrey,BrendanM;Xu,Jia-Rong;Rockey,DanielD;Rohrmann,GeorgeF;Jin,Ling
利用高通量DNA测序技术和引物走步技术对兔高致病性疱疹病毒4型(LHV-4)的基因组进行了分析。通过限制性内切酶酶切和Southern印迹分析进一步验证了所组装的DNA序列。LHV-4基因组全长约为124kb。LHV-4基因组中编码的基因与单纯疱疹病毒属的疱疹病毒关系最密切，包括人类疱疹病毒(HHV-1和HHV-2)、猴疱疹病毒包括脑疱疹病毒(CeHV-2和CeHV-16)、猕猴(McHV-1)、牛疱疹病毒2(BHV-2)和袋鼠(巨足)疱疹病毒的谱系(mahv-1和-2)。与其他单纯疱疹病毒基因组相似，LHV-4在独特区的G+C总含量高达65-70%，在反向重复区的总G+C含量为75-77%。在LHV-4基因组中未发现ICP34.5和US5的同源基因。这项研究表明，LHV-4拥有迄今为止最小的单纯疱疹病毒基因组。
The genome of a herpesvirus highly pathogenic to rabbits, leporid herpesvirus 4 (LHV-4), was analyzed using high-throughput DNA sequencing technology and primer walking. The assembled DNA sequences were further verified by restriction endonuclease digestion and Southern blot analyses. The total length of the LHV-4 genome was determined to be about 124kb. Genes encoded in the LHV-4 genome are most closely related to herpesvirus of the Simplexvirus genus, including human herpesviruses (HHV-1 and HHV-2), monkey herpesviruses including cercopithicine (CeHV-2 and CeHV-16), macacine (McHV-1), bovine herpesvirus 2 (BHV-2), and a lineage of wallaby (macropodid) herpesviruses (MaHV-1 and -2). Similar to other simplexvirus genomes, LHV-4 has a high overall G+C content of 65–70% in the unique regions and 75–77% in the inverted repeat regions. Orthologs of ICP34.5 and US5 were not identified in the LHV-4 genome. This study shows that LHV-4 has the smallest simplexvirus genome characterized to date.