Evidence for a rhesus monkey model of asymptomatic tuberculosis
Evidence for a rhesus monkey model of asymptomatic tuberculosis
复制标题
DOI：
10.1111/j.1600-0684.2004.00062.x
复制
发表时间：
2004-06-01
影响因子：
0.7
通讯作者：
Didier, PJ
中科院分区：
文献类型：
--
作者：
Gormus, BJ;Blanchard, JL;Didier, PJ
恒河猴（RM）支气管内接种不同剂量的结核分枝杆菌（MTB）菌株Erdman和H37 Rv，以建立无症状结核感染模型。Erdman菌株在7-11周内产生活动性疾病，与剂量无关。低剂量的H37 Rv导致无症状感染;高剂量在11周内产生活动性疾病。在4个月的接种后研究期间，MTB培养滤液蛋白（CFP）刺激的支气管肺泡灌洗细胞（BALC）和来自患有活动性疾病（30 cfu Erdman接种）或无症状感染（200 cfu H37 Rv接种）的猴的血液单核细胞（PBMC）产生类似的显著量的编码IFN-γ或TNF-α的mRNA，但IL-4 mRNA的量不显著。与无症状MTB感染相比，在活动性MTB感染动物中观察到抗原诱导的体外胚细胞生成反应和血清抗脂阿拉伯甘露聚糖（LAM）抗体反应的差异。结果表明，RM是使用低剂量H37 Rv研究无症状结核感染的良好模型。
Rhesus monkeys (RM) were inoculated intrabronchially with graded doses of Mycobacterium tuberculosis (MTB) strains Erdman and H37Rv in an effort to produce a model of asymptomatic tuberculosis infection. Erdman strain produced active disease within 7-11 weeks regardless of dose. Low doses of H37Rv resulted in asymptomatic infections; high doses produced active disease within 11 weeks. Over a 4-month period of post-inoculation study, MTB culture-filtrate protein (CFP)-stimulated bronchoalveolar lavage cells (BALC) and blood mononuclear cells (PBMC) from monkeys with active disease (30 cfu Erdman-inoculated) or asymptomatic infection (200 cfu H37Rv-inoculated) produced similar significant quantities of mRNA encoding for IFN-gamma or TNF-alpha, but insignificant quantities of IL-4 mRNA. Differences were observed in antigen-induced in vitro blastogenic responses and serum anti-lipoarabinomannan (LAM) antibody responses in animals with active compared with asymptomatic MTB infections. The results indicate that RM are a good model for the study of asymptomatic tuberculosis infections using low doses of H37Rv.