OPLA Scaffold, Collagen I, and Horse Serum Induce a Higher Degree of Myogenic Differentiation of Adult Rat Cardiac Stem Cells
OPLA Scaffold, Collagen I, and Horse Serum Induce a Higher Degree of Myogenic Differentiation of Adult Rat Cardiac Stem Cells
复制标题
DOI：
10.1002/jcp.21912
复制
发表时间：
2009-12-01
影响因子：
5.6
通讯作者：
Zummo, Giovanni
中科院分区：
文献类型：
--
作者：
Di Felice, Valentina;Ardizzone, Nella Maria;Zummo, Giovanni
过去几年，心脏研究的一个主要目标是驱动干细胞分化以替代受损的心肌。几个研究小组尝试使用补充有生长因子或作为分化物质的分子的二维或三维系统来区分潜在的心脏干细胞（CSC）。我们假设这些系统未能引发完全分化，因为它们缺乏架构空间。在本研究中，我们从成年大鼠心肌中分离出一组小型增殖和成纤维细胞样细胞。评估这些细胞的表型，并将表征的细胞在含有马血清的胶原蛋白 I/OPLA 支架中培养，以获得精细的心肌分化。当创建更类似于 CSC 生态位的环境时，C-Kit(POS)/Sca-1(POS) CSC 在体外完全分化。这些实验为研究 CSC 生物学和导致心肌分化的生化途径提供了一个重要模型。结果为新的手术方法铺平了道路。 J.细胞。生理学。 221: 729-739, 2009。(C) 2009 Wiley-Liss, Inc.
In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold with horse serum to obtain fine myocardial differentiation. C-Kit(POS)/Sca-1(POS) CSCs fully differentiated in vitro when an environment more similar to the CSC niche was created. These experiments demonstrated an important model for the study of the biology of CSCs and the biochemical pathways that lead to myocardial differentiation. The results pave the way for a new surgical approach. J. Cell. Physiol. 221: 729-739, 2009. (C) 2009 Wiley-Liss, Inc.