The impact of theaflavins on systemic-and microcirculation alterations: The murine and randomized feasibility trials
The impact of theaflavins on systemic-and microcirculation alterations: The murine and randomized feasibility trials
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DOI：
10.1016/j.jnutbio.2016.01.012
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发表时间：
2016-06-01
影响因子：
5.6
通讯作者：
Osakabe, Naomi
中科院分区：
文献类型：
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作者：
Saito, Akiko;Nakazato, Risa;Osakabe, Naomi
茶黄素是红茶中的多酚类物质；它们的生理活性尚未得到很好的研究。本研究评价了茶黄素对大鼠血液循环的影响。此外，一项干预试点研究检查了茶黄素饮料对餐后血流动力学变化的影响。在一项动物研究中，单次口服富含茶黄素的部分（TF, 10 mg/kg）引起平均血压（MBP）和心率（HR）的短暂性升高。TF也显著提高了胸血流量，其影响程度依次为：茶黄素3′- o -没食子酸酯(TF2B) > >茶黄素3′- o -没食子酸酯（TF2A）茶黄素(TF1) =茶黄素3,3′-二o -没食子酸酯（TF3）。此外，在哺乳动物中，用卡维地洛作为肾上腺素阻滞剂进行预处理后，这些血流动力学改变完全消失。同时给予大鼠10 mg/kg/d的TF治疗2周。在摄食期结束时，与蒸馏水相比，反复摄食TF显著降低了MBP，并升高了主动脉eNOS水平。在干预试验中，志愿者在摄入TF饮料（45 mg/杯）2 h和4 h后血压较治疗前显著升高。在摄入后4小时，安慰剂组和茶黄素组在口蹄疫方面有显著差异。这些结果表明茶黄素在小鼠和健康受试者中都有改变血液动力学的有效活性。需要进一步的研究来阐明细节；然而，动物实验结果表明，交感神经系统可能参与了TF引起的血流动力学改变。(C) 2016 Elsevier Inc.版权所有。
Theaflavins are polyphenols found in black tea; their physiological activities were not well investigated. The present study in rats evaluated the influence of theaflavins on circulation. In addition, an intervention pilot study examined the influence of a theaflavin drink on postprandial hemodynamic change. In an animal study, a single oral dose of theaflavin rich fraction (TF, 10 mg/kg) caused transient increase in mean blood pressure (MBP) and heart rate (HR). TF also elevated cremastric blood flow significantly, and the magnitude of this effect was in this order: theaflavin 3'-O-gallate (TF2B) > > theaflavin-3-O-gallate (TF2A) theaflavin (TF1) = theaflavin-3, 3'-di-O-gallate (TF3). In addition, these hemodynamic alterations in mammals totally disappeared when pretreated with carvedilol as an adrenaline blocker. We also treated 10-mg/kg/day TF to the rats for 2 weeks. At the end of the ingestion period, MBP was reduced significantly, and aortic eNOS level was elevated by the repeated ingestion of TF compared with distilled water. In the intervention trial, blood pressure of the volunteers was increased significantly 2 and 4 h after ingestion of the TF drink (45 mg/drink) compared with before treatment. A significant difference was observed in FMD between the placebo and theaflavin groups 4 h after ingestion. These results suggested that theaflavin has potent activity to alter hemodynamics in both murine and healthy subjects. Further studies is needed to elucidate the details; however, the results of animal study suggested that the possible involvement of sympathetic nervous system in the hemodynamic changes caused by TF. (C) 2016 Elsevier Inc. All rights reserved.