EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A COMPONENT OF A CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE
EVIDENCE THAT THE MAJOR POSTSYNAPTIC DENSITY PROTEIN IS A COMPONENT OF A CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE
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DOI：
10.1073/pnas.81.3.945
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
GREENGARD, P
中科院分区：
文献类型：
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作者：
KELLY, PT;MCGUINNESS, TL;GREENGARD, P
分离的[大鼠]突触连接中分子量为50,000和60,000的多肽与Ca2+/钙调素依赖性蛋白激酶II中相应分子量的多肽进行了比较。从两种制剂中得到的相应分子量的多肽通过几种标准显示为难以区分。这些标准包括125i标记的胰蛋白酶/凝乳胰蛋白酶模式，32p标记的蛋白水解肽图，以及使用多克隆和单克隆抗体在免疫印迹上的交叉反应性。内源性突触连接激酶和Ca2+/钙调素依赖性蛋白激酶II对底物蛋白磷酸化的研究表明，这两种酶具有相似的底物特异性。由于已知存在于突触连接中的mw50,000多肽是主要的突触后密度蛋白，因此结果表明主要的突触后密度蛋白是Ca2+/钙调素依赖性蛋白激酶II的一个组成部分。
Polypeptides of MW 50,000 and 60,000 in isolated [rat] synaptic junctions have been compared to polypeptides of corresponding MW in Ca2+/calmodulin-dependent protein kinase II. The polypeptides of corresponding MW from the 2 preparations were shown by several criteria to be indistinguishable. These criteria included 125I-labeled tryptic/chymotryptic peptide patterns, 32P-labeled proteolytic peptide maps, and crossreactivity on immunoblots using polyclonal and monoclonal antibodies. Studies examining the phosphorylation of substrate proteins, by the endogenous synaptic junction kinase and by Ca2+/calmodulin-dependent protein kinase II, indicated that the 2 enzymes have similar substrate specificities. Since the MW 50,000 polypeptide present in synaptic junctions is known to be the major postsynaptic density protein, the results indicate that the major postsynaptic density protein is a component of Ca2+/calmodulin-dependent protein kinase II.