The degradation of the three layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane in vitro
The degradation of the three layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane in vitro
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DOI：
10.1016/j.dental.2006.06.045
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发表时间：
2007-09-01
期刊：
影响因子：
5
通讯作者：
Cui, Fuzhai
中科院分区：
文献类型：
--
作者：
Liao, Susan;Watari, Furnio;Cui, Fuzhai
目标。研究了纳米碳化羟基磷灰石/胶原/聚乳酸-羟基乙酸共聚物(nCHAC/PLGA)引导组织再生复合膜的体外生物降解性。特别针对牙周治疗，制备了功能梯度材料(FGM)nCHAC/PLGA膜，膜由3层组成，其中8wt%nCHAC+PLGA/4wt%nCHAC+PLGA/PLGA，膜的一侧是多孔的，使细胞生长在膜的对侧表面光滑，从而抑制细胞的黏附。将nCHAC/PLGA体外降解标本分别在37℃人工唾液中浸泡1、2、4、8、12周，检测其在此期间的失重情况，并以纯PLGA膜为对照，比较其降解行为。测定残液的pH值和钙浓度，并用扫描电子显微镜(SEM)观察其形态变化。在体外实验期间，膜的整体形状可保持4周，之后在8~12周时为粉末状。结果表明，nCHAC/PLGA三层功能梯度复合膜的质量损失持续增加，4周后质量下降23.1%，12周后下降88%。4周后残液中钙离子浓度明显升高，说明纳米碳化羟基磷灰石发生了降解。此外，nCHAC/PLGA膜的溶液pH值略高于纯PLGA膜，这表明nCHAC复合材料对PLGA在溶液中的酸化产物可能有中和作用。8wt%nCHAC+PLGA的孔结构在多孔表面增大，而纯PLGA的非孔表面也随着时间的延长而呈现出较小的孔结构。复合膜的降解是适合实际牙周修复的。此外，复合膜表面形成的新的矿物质可能对体内新骨组织的再生有积极作用。(C)2006年牙科材料学会。爱思唯尔有限公司出版。保留所有权利。
Objective. The purpose of this paper was to investigate the in vitro biodegradation of a guided tissue regeneration composite membrane, nano-carbonated hydroxyapatite/collagen/ poly(lactic - co- glycolic acid) (nCHAC/PLGA). Especially for periodontal therapy, the functional graded material (FGM) nCHAC/PLGA membrane was prepared that consisted of three layers with 8 wt% nCHAC + PLGA/4 wt% nCHAC + PLGA/PLGA, where one face of the membrane is porous, thereby allowing cell growth thereon and the opposite face of the membrane smooth, thereby inhibiting cell adhesion.Methods. For evaluation, in vitro degradation specimens of nCHAC/PLGA were immersed into artificial saliva solution at 37 degrees C for 1, 2, 4, 8 and 12 weeks to detect the weight loss over the period, and set pure PLGA membrane as control to compare the degraded behaviors. pH value and calcium concentration of the residual solution were measured, and morphology change was investigated by scanning electron microscopy (SEM).Results. During the experimental period in vitro, the whole shape of the membrane could be kept for 4 weeks, after that it became powder at between 8 and 12 weeks. The results demonstrated that weight loss increased continuously with a reduction in mass of 23.1% after 4 weeks and 88% after 12 week for the nCHAC/PLGA three FGM layers composite membrane. The calcium concentration in the residual solution showed a significant increase after 4 weeks, which referred to the nano-carbonated hydroxyapatite degradation. Moreover, the pH value in the solution of the nCHAC/PLGA membrane was a little higher than that of the pure PLGA membrane, which demonstrated the possible neutralization effect from nCHAC composite for the acid outcome of PLGA in the solution. The pore structure of 8 wt% nCHAC + PLGA was enlarged on the porous surface, while the nonporous surface of pure PLGA also showed a small porous structure after increased time.Significance. Degradation of the composite membrane is appropriate for practical periodontal repair. Moreover, the new mineral formation on the surface of the composite membrane referred to the possible positive effect in vivo for new bone tissue regeneration. (C) 2006 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.