A modular yeast biosensor for low-cost point-of-care pathogen detection.
A modular yeast biosensor for low-cost point-of-care pathogen detection.
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DOI：
10.1126/sciadv.1603221
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发表时间：
2017-06
期刊：
影响因子：
13.6
通讯作者：
Cornish VW
中科院分区：
文献类型：
--
作者：
Ostrov N;Jimenez M;Billerbeck S;Brisbois J;Matragrano J;Ager A;Cornish VW
基于酵母的试纸生物传感器能够实现低成本的病原体现场监测。简单、特异和廉价的现场检测方法的可用性对于病原体监测网络的部署至关重要。我们开发了一种非技术性且高度特异性的比色法，用于检测基于酿酒酵母（一种遗传上易于处理的模式生物和家用产品）的病原体衍生肽。整合G蛋白偶联受体与一个可见的，无试剂的番茄红素读出，我们展示了主要的人类，植物和食品真菌病原体与纳摩尔灵敏度的差异检测。我们进一步优化了一步快速试纸原型，可用于复杂样品，包括血液，尿液和土壤。这种模块化生物传感器可以大规模经济地生产，不依赖于冷链储存，可以在没有额外设备的情况下进行检测，因此是一个可扩展到全球病原体监测的引人注目的平台。
A yeast-based dipstick biosensor enables low-cost, on-site surveillance of pathogens. The availability of simple, specific, and inexpensive on-site detection methods is of key importance for deployment of pathogen surveillance networks. We developed a nontechnical and highly specific colorimetric assay for detection of pathogen-derived peptides based on Saccharomyces cerevisiae—a genetically tractable model organism and household product. Integrating G protein–coupled receptors with a visible, reagent-free lycopene readout, we demonstrate differential detection of major human, plant, and food fungal pathogens with nanomolar sensitivity. We further optimized a one-step rapid dipstick prototype that can be used in complex samples, including blood, urine, and soil. This modular biosensor can be economically produced at large scale, is not reliant on cold-chain storage, can be detected without additional equipment, and is thus a compelling platform scalable to global surveillance of pathogens.