Transcriptome Analysis of Salt-Sensitive and Tolerant Genotypes Reveals Salt-Tolerance Metabolic Pathways in Sugar Beet
Transcriptome Analysis of Salt-Sensitive and Tolerant Genotypes Reveals Salt-Tolerance Metabolic Pathways in Sugar Beet
复制标题
盐敏感和耐盐基因型的转录组分析揭示了甜菜的耐盐代谢途径
DOI：
10.3390/ijms20235910
复制
发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Wang, Yuguang
中科院分区：
文献类型：
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作者：
Geng, Gui;Lv, Chunhua;Wang, Yuguang
土壤盐渍化是一种常见的环境问题，严重影响农作物的产量和品质。甜菜（Beta vulgaris L.），作为世界上主要的糖料作物之一，其耐盐性很强。为了阐明甜菜盐胁迫的分子机制，我们对两种不同的甜菜基因型进行了转录组学分析。据我们所知，这是第一次比较盐反应转录组在甜菜与对比基因型。与盐敏感品种（S710）相比，耐盐品种（T710MU）表现出更好的生长，并表现出更高的叶绿素含量，更高的抗氧化酶活性，和渗透调节分子的水平增加。利用高通量实验系统，在盐敏感基因型和耐盐基因型的叶片中分别鉴定出1714和2912个差异表达基因。许多差异表达的基因涉及胁迫和防御反应、代谢过程、信号转导、运输过程和细胞壁合成。此外，几个基因的表达模式不同的两个品种在盐胁迫下，和几个关键途径参与决定甜菜的耐盐性，被确定。本研究揭示了甜菜耐盐的机理，为培育耐盐品种提供了潜在的代谢途径和基因标记。
Soil salinization is a common environmental problem that seriously affects the yield and quality of crops. Sugar beet (Beta vulgaris L.), one of the main sugar crops in the world, shows a strong tolerance to salt stress. To decipher the molecular mechanism of sugar beet under salt stress, we conducted transcriptomic analyses of two contrasting sugar beet genotypes. To the best of our knowledge, this is the first comparison of salt-response transcriptomes in sugar beet with contrasting genotypes. Compared to the salt-sensitive cultivar (S710), the salt-tolerant one (T710MU) showed better growth and exhibited a higher chlorophyll content, higher antioxidant enzyme activity, and increased levels of osmotic adjustment molecules. Based on a high-throughput experimental system, 1714 differentially expressed genes were identified in the leaves of the salt-sensitive genotype, and 2912 in the salt-tolerant one. Many of the differentially expressed genes were involved in stress and defense responses, metabolic processes, signal transduction, transport processes, and cell wall synthesis. Moreover, expression patterns of several genes differed between the two cultivars in response to salt stress, and several key pathways involved in determining the salt tolerance of sugar beet, were identified. Our results revealed the mechanism of salt tolerance in sugar beet and provided potential metabolic pathways and gene markers for growing salt-tolerant cultivars.