A convenient method for synthesis of glyconanoparticles for colorimetric measuring carbohydrate-protein interactions.
A convenient method for synthesis of glyconanoparticles for colorimetric measuring carbohydrate-protein interactions.
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DOI：
10.1016/j.bbrc.2009.08.079
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发表时间：
2009-11-06
影响因子：
3.1
通讯作者：
Turchi, Craig
中科院分区：
文献类型：
--
作者：
Chuang, Yen-Jun;Zhou, Xichun;Pan, Zhengwei;Turchi, Craig
关键词：
碳水化合物官能化的纳米颗粒，即，糖纳米颗粒具有广泛的应用，包括碳水化合物-蛋白质相互作用的研究、体内细胞成像、生物标记等。目前报道的制备糖纳米颗粒的方法需要对碳水化合物部分进行多步修饰以缀合到纳米颗粒表面。然而，所需的合成操作是困难和耗时的。本文报道了一种简单而通用的制备糖纳米颗粒的方法。该方法基于利用清洁且方便的微波辐射能量将未修饰的碳水化合物一步、位点特异性缀合到酰肼官能化的Au纳米颗粒上。还提出了利用金糖纳米颗粒和伴刀豆球蛋白A（ConA）的集合的比色测定。建立了一个可行的分析体系，用于分析凝集素与五种Au糖纳米颗粒的多价相互作用，并测定了它们的解离常数。测量了Au糖聚糖颗粒表面等离子体激元随凝集素-碳水化合物相互作用的变化，并基于非线性曲线拟合确定了解离常数。发现碳水化合物与ConA的相互作用的强度如下：麦芽糖>甘露糖>葡萄糖>乳糖> MAN 5。
Carbohydrate functionalized nanoparticles, i.e., the glyconanoparticles, have wide application ranging from studies of carbohydrate-protein interactions, in vivo cell imaging, biolabeling, etc. Currently reported methods for preparation of glyconanoaprticles require multi-step modifications of carbohydrates moieties to conjugate to nanoparticle surface. However, the required synthetic manipulations are difficult and time consuming. We report herewith a simple and versatile method for preparing glyconanoparticles. This method is based on the utilization of clean and convenient microwave irradiation energy for one-step, site-specific conjugation of unmodified carbohydrates onto hydrazide-functionalized Au nanoparticles. A colorimetric assay that utilizes the ensemble of gold glyconanoparticles and Concanavalin A (ConA) was also presented. This feasible assay system was developed to analyze multivalent interactions and to determine the dissociation constant (Kd) for five kind of Au glyconanoparticles with lectin. Surface plasmon changes of the Au glyconanparticles as a function of lectin-carbohydrate interactions were measured and the dissociation constants were determined based on non-linear curve fitting. The strength of the interaction of carbohydrates with ConA was found to be as follows: Maltose > Mannose > Glucose > Lactose > MAN5.
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影响因子：
3.1
作者：
Ojala, CR;Ostman, JM;Ojala, WH
通讯作者：
Ojala, WH
影响因子：
64.8
作者：
Kitov, PI;Sadowska, JM;Bundle, DR
通讯作者：
Bundle, DR
影响因子：
3.9
作者：
Schofield, Claire L.;Haines, Alan H.;Russell, David A.
通讯作者：
Russell, David A.
DOI：
10.1073/pnas.94.20.10554
发表时间：
1997-09-30
影响因子：
11.1
作者：
Liang, R;Loebach, J;Kahne, D
通讯作者：
Kahne, D
影响因子：
3.1
作者：
Ojeda, Rafael;de Paz, Jose Luis;Penades, Soledad
通讯作者：
Penades, Soledad