VIRAL-ENCODED SMALL RNAS IN HERPESVIRUS SAIMIRI INDUCED-TUMORS
VIRAL-ENCODED SMALL RNAS IN HERPESVIRUS SAIMIRI INDUCED-TUMORS
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DOI：
10.1002/j.1460-2075.1986.tb04405.x
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发表时间：
1986-07-01
期刊：
影响因子：
11.4
通讯作者：
DESROSIERS, RC
中科院分区：
文献类型：
--
作者：
MURTHY, S;KAMINE, J;DESROSIERS, RC
疱疹病毒L左侧末端的序列对病毒的致癌和转化潜能是必不可少的，但这些序列不是复制所必需的。用Northern杂交和核酸酶保护分析研究了来源于疱疹病毒SAIMIRI基因组0.0~6.7个核苷酸单位(7.4kbp)的RNA。虽然在体外允许感染的单层细胞中检测到几个含Poly(A)的RNA，但这些RNA不能在直接从病毒诱导的淋巴瘤细胞中检测到，也不能在淋巴母细胞瘤细胞系1670中检测到。相反，这些转化的T细胞表达来自该区域的大约73、105、110和135个核苷酸的四个小RNA。在单层细胞裂解感染过程中未检测到这些小RNA。因此，这些RNA的合成以细胞类型特定的方式受到严格调控。每个小RNA的基因组编码序列被映射到0.5-1.2kbp的DNA片段，其长度超过4.3kbp的病毒遗传信息。这些发现以及该区域缺失突变体的生物学特性使我们推测，这些小RNA中的一个或多个在疱疹病毒saimiri的细胞生长转化中发挥重要作用。
DNA sequences from the left terminus of herpesvirus saimiri L-DNA are essential for the oncogenic and transforming potential of the virus, but these sequences are not required for replication. RNA derived from 0.0 to 6.7 map units (7.4 kbp) on the herpesvirus saimiri genome was studied by Northern blot hybridization and by nuclease protection analyses. Although several poly(A)-containing RNAs were detected from this region in permissively-infected monolayer cells in vitro, these RNAs could not be detected in cells taken directly from viral-induced lymphomas nor in the lymphoblastoid tumor cell line 1670. Instead, these transformed T-cells expressed four small RNAs of approximately 73, 105, 110 and 135 nt derived from this region. These small RNAs were not detected at all during the course of lytic infection of monolayer cells. Thus, synthesis of these RNAs is stringently regulated in a cell-type specific manner. Genomic coding sequences for each of these small RNAs were mapped to 0.5-1.2 kbp DNA fragments stretched over 4.3 kbp of viral genetic information. These findings together with the biological properteis of mutants with deletions in this region have led us to speculate that one or more of these small RNAs play an essential role in cell growth transformation by herpesvirus saimiri.