Genome-wide identification of Arabidopsis long noncoding RNAs in response to the blue light
Genome-wide identification of Arabidopsis long noncoding RNAs in response to the blue light
复制标题
拟南芥长非编码 RNA 响应蓝光的全基因组鉴定
DOI：
10.1038/s41598-020-63187-1
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发表时间：
2020-04-10
影响因子：
4.6
通讯作者：
Fang, Yuda
中科院分区：
文献类型：
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作者：
Sun, Zhenfei;Huang, Kai;Fang, Yuda
长链非编码RNA（lncRNA）在动物体内广泛参与多种生物学过程。在植物中，光作为一种重要的外部信号调节植物的生长和发育。然而，很少有人知道植物lncRNA在响应光的作用。在本研究中，我们对蓝光照射2 h和8 h的拟南芥幼苗中的信使RNA（mRNA）、lncRNA和microRNA（miRNA）进行了测序。与暗处理相比，蓝光处理2 h和8 h分别鉴定出4197个mRNA、375个miRNA和481个lncRNA，或5207个mRNA、286个miRNA和545个lncRNA的差异表达。随后，共构建了407个竞争性内源RNA（ceRNA）对（lncRNA-mRNA-miRNA）。我们发现了一种蓝光诱导的lncRNA，它在蓝光引导的植物光形态建成和对甘露醇胁迫的响应中发挥作用，作为一种ceRNA以一种靶模拟方式隔离miR 167。这些结果揭示了lncRNA在蓝光信号和甘露醇应激中的未知作用，并提供了与蓝光响应的miRNA相关的lncRNA的有用资源。
Long non-coding RNAs (lncRNAs) have been shown in animals to play roles in a wide range of biological processes. In plant, light modulates the growth and development as a key external signal. However, little is known about the role of plant lncRNA in response to light. In this study, we sequenced the messenger RNAs (mRNAs), lncRNAs and microRNAs (miRNAs) in Arabidopsis seedlings under blue light for 2 h and 8 h. Compared to dark, we identified 4197 mRNAs, 375 miRNAs and 481 lncRNAs, or 5207 mRNAs, 286 miRNAs and 545 lncRNAs of differential expressions under blue light treatments for 2 h or 8 h respectively. Subsequently, a total of 407 competing endogenous RNA (ceRNA) pairs (lncRNA-mRNA-miRNA) were constructed. We identified a blue light-induced lncRNA which plays roles in blue light-directed plant photomorphogenesis and response to mannitol stress by serving as a ceRNA to sequester miR167 in a type of target mimicry. These results revealed previously unknown roles of the lncRNA in blue light signaling and mannitol stress, and provided useful resources of lncRNAs associated with miRNAs in response to blue light.