Prokaryotic DNA segregation by an actin-like filament
Prokaryotic DNA segregation by an actin-like filament
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DOI：
10.1093/emboj/cdf320
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发表时间：
2002-06-17
期刊：
影响因子：
11.4
通讯作者：
Gerdes, K
中科院分区：
文献类型：
--
作者：
Moller-Jensen, J;Jensen, RB;Gerdes, K
原核生物 DNA 分离的机制很大程度上未知。大肠杆菌质粒 R1 编码的分配位点 (par) 主动将其复制子与子细胞分离。我们在这里展示了 par 编码的 ParM ATP 酶形成动态肌动蛋白样丝，具有力生成蛋白的预期特性。丝的形成取决于 par、ParR 和 ParR 结合的类着丝粒 parC 区域编码的其他成分。 ParM ATP酶缺陷的突变体表现出超丝状并且不支持质粒分配。 ParM 聚合依赖于 ATP，ParM 丝的解聚需要核苷酸水解。我们的体内和体外结果表明，ParM 聚合产生了质粒定向运动到相反细胞极所需的力，并且 ParR-parC 复合物充当 ParM 聚合的成核点。因此，我们为真核有丝分裂纺锤体的简单原核类似物提供了证据。
The mechanisms responsible for prokaryotic DNA segregation are largely unknown. The partitioning locus (par) encoded by the Escherichia coli plasmid R1 actively segregates its replicon to daughter cells. We show here that the ParM ATPase encoded by par forms dynamic actin-like filaments with properties expected for a force-generating protein. Filament formation depended on the other components encoded by par, ParR and the centromere-like parC region to which ParR binds. Mutants defective in ParM ATPase exhibited hyperfilamentation and did not support plasmid partitioning. ParM polymerization was ATP dependent, and depolymerization of ParM filaments required nucleotide hydrolysis. Our in vivo and in vitro results indicate that ParM polymerization generates the force required for directional movement of plasmids to opposite cell poles and that the ParR-parC complex functions as a nucleation point for ParM polymerization. Hence, we provide evidence for a simple prokaryotic analogue of the eukaryotic mitotic spindle apparatus.