Detection of amide I signals of interfacial proteins in situ using SFG.
Detection of amide I signals of interfacial proteins in situ using SFG.
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DOI：
10.1021/ja036373s
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发表时间：
2003-07
影响因子：
15
通讯作者：
Jie Wang;M. Even;Xiaoyun Chen;A. Schmaier;J. Waite;Zhan Chen
中科院分区：
文献类型：
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作者：
Jie Wang;M. Even;Xiaoyun Chen;A. Schmaier;J. Waite;Zhan Chen
In this Communication, we demonstrate the novel observation that it is feasible to collect amide signals from polymer/protein solution interfaces in situ using sum frequency generation (SFG) vibrational spectroscopy. Such SFG amide signals allow for acquisition of more detailed molecular level information of entire interfacial protein structures. Proteins investigated include bovine serum albumin, mussel protein mefp-2, factor XIIa, and ubiquitin. Our studies indicate that different proteins generate different SFG amide signals at the polystyrene/protein solution interface, showing that they have different interfacial coverage, secondary structure, or orientation.
