Artificial assembly of envelope particles of HVJ (Sendai virus). II. Lipid components for formation of the active hemolysin.
Artificial assembly of envelope particles of HVJ (Sendai virus). II. Lipid components for formation of the active hemolysin.
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HVJ（仙台病毒）包膜颗粒的人工组装。
DOI：
10.1016/0042-6822(72)90520-x
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发表时间：
1972
期刊：
影响因子：
3.7
通讯作者：
Y. Shimizu
中科院分区：
文献类型：
--
作者：
Y. Hosaka;Y. Shimizu
通过先前报告（Hocolate和Shimizu，1972）中所述的方法分离HVJ的上HA条带部分，并在Nonidet P40存在下与脂质混合，然后用PBS透析。在该系统中，研究了允许形成活性溶血素的脂质组分。当HVJ的四种主要磷脂组分分别使用时，磷脂酰乙醇胺是最有效的，磷脂酰胆碱和鞘磷脂仅在高浓度下有效，磷脂酰丝氨酸无效。来自商业来源的磷脂酰乙醇胺与来自病毒来源的磷脂酰乙醇胺一样有效。在混合的病毒磷脂中，发现了类似于协同效应的现象：低浓度的胆固醇促进活性溶血素的形成，而高浓度的胆固醇则抑制活性溶血素的形成。由此形成的活性溶血素出现大于600 μ m的大膜颗粒，具有刺突。在活性溶血素中未检测到磷脂酶活性。
The upper HA band fraction of HVJ was isolated by the method as described in the previous report (Hosaka and Shimizu, 1972) and mixed with lipids in the presence of Nonidet P40 and dialyzed against PBS. In this system, lipid components permitting formation of the active hemolysins were investigated. When the four major phospholipid components of HVJ were used separately, phosphatidylethanolamine was the most effective, phosphatidylcholine and sphingomyelin were effective only at high concentrations, and phosphatidylserine was ineffective. Phosphatidylethanolamine from commercial sources was as effective as that from viral sources. In mixed viral phospholipids, something like cooperative effectiveness was found.A low concentration of cholesterol stimulated the formation of active hemolysins, but a high concentration inhibited it. Cholesterol together with phospholipids seemed to contribute to the rigidity of the membranes. Thus formed active hemolysins appeared large membranous particles of more than 600 Å with spikes. No phospholipase activity was detected in the active hemolysins.