Arabidopsis TAF1 is an MRE11-interacting protein required for resistance to genotoxic stress and viability of the male gametophyte.
Arabidopsis TAF1 is an MRE11-interacting protein required for resistance to genotoxic stress and viability of the male gametophyte.
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拟南芥 TAF1 是一种 MRE11 相互作用蛋白，是抵抗遗传毒性应激和雄性配子体活力所必需的。
DOI：
10.1111/tpj.13020
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
West CE
中科院分区：
文献类型：
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作者：
Waterworth WM;Drury GE;Blundell-Hunter G;West CE
通过重组途径修复DNA双链断裂（DSB）对植物生长和生育力至关重要。重组核酸内切酶MRE 11在DNA双链断裂的检测和修复中起着重要作用。在这里，我们证明了拟南芥MRE 11和组蛋白乙酰转移酶TAF 1之间的蛋白质相互作用，TAF 1是RNA聚合酶II转录起始因子复合物TFIID的TATA结合蛋白相关因子（TAF）。拟南芥有两个TAF 1同源物，称为TAF 1和TAF 1b，突变体taf 1b系是可行的和可育的。相反，taf 1无效突变是致命的，表明TAF 1是一个必需的基因。杂合的taf 1 +/−植物显示由花粉管发育缺陷引起的突变等位基因的异常分离，表明TAF 1对配子活力很重要。一个等位基因系列的taf 1线的表征显示，亚纯型突变体是可行的，但显示发育缺陷和降低植物生育力。缺乏C-末端溴结构域的taf 1突变体对X射线和丝裂霉素C的超敏性，但对其他形式的非生物胁迫不敏感，建立了TAF 1在植物DNA修复过程中的特定作用。总的来说，这些研究揭示了TAF 1在植物抗遗传毒性胁迫中的功能，为植物DNA损伤反应的分子机制提供了进一步的见解。 DNA重组途径对生物体的基因组稳定性、繁殖力和生存至关重要。在这里，我们建立了一个重组核酸内切酶和组蛋白乙酰转移酶参与转录起始之间的分子联系，提供深入了解染色体断裂检测和DNA修复过程。
Repair of DNA double‐strand breaks (DSBs) by recombination pathways is essential for plant growth and fertility. The recombination endonuclease MRE11 plays important roles in sensing and repair of DNA DSBs. Here we demonstrate protein interaction between Arabidopsis MRE11 and the histone acetyltransferase TAF1, a TATA‐binding protein Associated Factor (TAF) of the RNA polymerase II transcription initiation factor complex TFIID. Arabidopsis has two TAF1 homologues termed TAF1 and TAF1b and mutant taf1b lines are viable and fertile. In contrast, taf1 null mutations are lethal, demonstrating that TAF1 is an essential gene. Heterozygous taf1 +/− plants display abnormal segregation of the mutant allele resulting from defects in pollen tube development, indicating that TAF1 is important for gamete viability. Characterization of an allelic series of taf1 lines revealed that hypomorphic mutants are viable but display developmental defects and reduced plant fertility. Hypersensitivity of taf1 mutants lacking the C‐terminal bromodomain to X‐rays and mitomycin C, but not to other forms of abiotic stress, established a specific role for TAF1 in plant DNA repair processes. Collectively these studies reveal a function for TAF1 in plant resistance to genotoxic stress, providing further insight into the molecular mechanisms of the DNA damage response in plants. DNA recombination pathways are critical for genome stability, fertility and survival of organisms. Here we establish a molecular link between a recombination endonuclease and a histone acetyltransferase involved in transcription initiation, providing insight into chromosomal break detection and DNA repair processes.