Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.
Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.
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干涉反射成像传感器（IRIS） - 一种用于多重诊断和数字检测的平台技术。
DOI：
10.3390/s150717649
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发表时间：
2015-07-20
期刊：
影响因子：
--
通讯作者：
Ünlü MS
中科院分区：
文献类型：
--
作者：
Avci O;Ünlü NL;Özkumur AY;Ünlü MS
在过去的十年中，对疾病诊断的日益增长的需求刺激了具有前所未有能力的新技术的快速发展。最近新出现的传染病和流行病揭示了现有诊断工具的缺点，以及进一步改进的必要性。光学生物传感器通过提供以高灵敏度、特异性、定量和多重方式检测生物标志物的手段，为下一代诊断奠定了基础。在这里，我们回顾了一种光学传感技术，干涉反射成像传感器(IRIS)，以及这个用于定量、无标记和动态检测的多功能平台的相关特征。我们讨论了IRIS的两种不同的模式：(I)低放大(整体生物分子质量测量)和(Ii)高放大(单个纳米颗粒的数字检测)及其应用，包括多个蛋白质芯片的无标记检测、单核苷酸多态性的测量、转录因子DNA结合的量化以及纳米颗粒和病毒的高灵敏度数字传感和表征。
Over the last decade, the growing need in disease diagnostics has stimulated rapid development of new technologies with unprecedented capabilities. Recent emerging infectious diseases and epidemics have revealed the shortcomings of existing diagnostics tools, and the necessity for further improvements. Optical biosensors can lay the foundations for future generation diagnostics by providing means to detect biomarkers in a highly sensitive, specific, quantitative and multiplexed fashion. Here, we review an optical sensing technology, Interferometric Reflectance Imaging Sensor (IRIS), and the relevant features of this multifunctional platform for quantitative, label-free and dynamic detection. We discuss two distinct modalities for IRIS: (i) low-magnification (ensemble biomolecular mass measurements) and (ii) high-magnification (digital detection of individual nanoparticles) along with their applications, including label-free detection of multiplexed protein chips, measurement of single nucleotide polymorphism, quantification of transcription factor DNA binding, and high sensitivity digital sensing and characterization of nanoparticles and viruses.
影响因子：
4.4
作者：
Hessner MJ;Singh VK;Wang X;Khan S;Tschannen MR;Zahrt TC
通讯作者：
Zahrt TC