Viral infection: CRISPR-Cas enhances HGT by transduction.
Viral infection: CRISPR-Cas enhances HGT by transduction.
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DOI：
10.1038/nrmicro.2018.28
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发表时间：
2018-04-01
期刊：
影响因子：
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通讯作者：
Du Toit, Andrea
中科院分区：
文献类型：
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作者：
Du Toit, Andrea
细菌水平基因转移（HGT）的主要机制是自然转化、接合和转导（噬菌体介导的细菌DNA转移）。除了在噬菌体抗性中发挥作用外，CRISPR-Cas 系统还被证明可以抑制接合和转化，但它们在转导中的作用尚不清楚。沃森等人。研究表明，在黑脓杆菌中，噬菌体介导的质粒、染色体位点和基因组岛的转导可以受到 CRISPR-Cas 干扰的限制。然而，由于与噬菌体中的间隔区相比，转导过程中获得的间隔区频率较低，因此他们假设 CRISPR-Cas 在噬菌体抗性中的典型作用将通过增加转导子的存活率来增强 HGT。事实上，从噬菌体获得间隔区使 CRISPR-Cas 能够保护细菌免受感染并增加转导子的产生。最后，细菌通过转导获得了染色体 CRISPR-Cas 系统。
The major mechanisms of horizontal gene transfer (HGT) in bacteria are natural transformation, conjugation and transduction (phage-mediated transfer of bacterial DNA). In addition to a role in phage resistance, CRISPR–Cas systems have been shown to inhibit conjugation and transformation, whereas their role in transduction is not well understood. Watson et al. show that in Pectobacterium atrosepticum, phage-mediated transduction of plasmids, chromosomal loci and genomic islands can be limited by CRISPR–Cas interference. However, owing to the infrequency of spacers acquired during transduction compared with spacers from phages, they hypothesized that the canonical role of CRISPR–Cas in phage resistance would enhance HGT by increasing the survival of transductants. Indeed, spacer acquisition from phages enabled CRISPR–Cas to protect bacteria from infection and increased the generation of transductants. Finally, bacteria acquired a chromosomal CRISPR–Cas system through transduction.