Inhibition of experimental corneal neovascularisation by bevacizumab (Avastin)
Inhibition of experimental corneal neovascularisation by bevacizumab (Avastin)
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DOI：
10.1136/bjo.2006.107912
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Chevez-Barrios, Patricia
中科院分区：
文献类型：
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作者：
Manzano, Roberta P. A.;Peyman, Gholam A.;Chevez-Barrios, Patricia
目的：评价局部应用贝伐单抗（Avastin）对大鼠实验性角膜新生血管形成的影响。方法：采用银硝酸盐棒（75%银，25%硝酸钾）对16只雄性Long Evans大鼠16只眼角膜进行化学烧灼。在接下来的7天中，治疗组中的10只眼睛滴注贝伐珠单抗4 mg/ml滴眼液，每日两次，而对照组中的6只眼睛接受安慰剂（生理盐水滴眼液，每日两次）。对角膜的数码照片进行分析，以确定新生血管覆盖的角膜面积占总角膜面积的百分比。在贝伐珠单抗治疗的眼睛中，新生血管平均覆盖38.2%（百分之十五点五）（平均值（SD）），与63.5%（5.0%）对照组（p < 0.02，Mann-Whitney U检验）。在雄性Long Evans大鼠模型中，局部给予浓度为4 mg/ml的贝伐珠单抗（Avastin）可限制化学损伤后的角膜新生血管形成。
Aim: To evaluate the effect of topically administered bevacizumab (Avastin) on experimental corneal neovascularisation in rats.Methods: Silver nitrate sticks (75% silver nitrate, 25% potassium nitrate) were used to perform chemical cauterisation on the corneas of 16 eyes from 16 male Long Evans rats. For the following 7 days, the 10 eyes in the treatment group were instilled with bevacizumab 4 mg/ml drops twice daily, whereas the 6 eyes in the control group received placebo ( normal saline drops twice daily). Digital photographs of the cornea were analysed to determine the area of cornea covered by neovascularisation as a percentage of the total corneal area.Results: In the bevacizumab-treated eyes, neovascularisation covered, on average, 38.2% (15.5%) ( mean (SD)) of the corneal surface compared with 63.5% (5.0%) in the control group (p < 0.02, Mann-Whitney U test).Conclusion: Topically administered bevacizumab ( Avastin) at a concentration of 4 mg/ml limits corneal neovascularisation following chemical injury in the male Long Evans rat model.