CHARACTERIZATION OF A NEUTRAL POLYSACCHARIDE HAVING ACTIVITY ON THE RETICULOENDOTHELIAL SYSTEM FROM THE RHIZOME OF CURCUMA-LONGA
CHARACTERIZATION OF A NEUTRAL POLYSACCHARIDE HAVING ACTIVITY ON THE RETICULOENDOTHELIAL SYSTEM FROM THE RHIZOME OF CURCUMA-LONGA
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DOI：
10.1248/cpb.40.185
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发表时间：
1992-01-01
影响因子：
1.7
通讯作者：
TOMODA, M
中科院分区：
文献类型：
--
作者：
GONDA, R;TAKEDA, K;TOMODA, M
从姜黄（Curcuma longa L.）其在电泳和凝胶色谱上均为单一条带，分子量估计为28000。它在碳清除试验中显示出显著的网状内皮系统增强活性。Ukonan D由摩尔比为1：1：12：0.2的L-阿拉伯糖：D-半乳糖：D-葡萄糖：D-甘露糖以及少量肽部分组成。甲基化分析、碳13核磁共振和酶降解研究表明，其结构特征主要包括α-1，5-连接的L-阿拉伯-β-3，6-分支的D-半乳聚糖型和α-4，6-分支的D-葡聚糖型结构单元。用α-淀粉酶降解，然后消除葡聚糖侧链对其免疫活性的影响进行了讨论。
A neutral polysaccharide, named ukonan D, was isolated from the rhizome of Curcuma longa L. It produced a single band on electrophoresis and a single peak on gel chromatography, and its molecular mass was estimated to be 28000. It showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test. Ukonan D is composed of L-arabinose:D-galactose:D-glucose:D-mannose in the molar ratio of 1:1:12:0.2, in addition to small amounts of peptide moiety. Methylation analysis, carbon-13 nuclear magnetic resonance and enzymic degradation studies indicated that its structural features include mainly both alpha-1,5-linked L-arabino-beta-3,6-branched D-galactan type and alpha-4,6-branched D-glucan type structural units. The influence of degradation with alpha-amylase followed by the elimination of glucan side chains on its immunological activity was discussed.