Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress
Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress
复制标题
拟南芥中玉米丝裂原激活蛋白激酶基因 ZmSIMK1 的过度表达增加了对盐胁迫的耐受性
DOI：
10.1007/s11033-010-0066-6
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发表时间：
2010-12-01
影响因子：
2.8
通讯作者：
Li, De-Quan
中科院分区：
文献类型：
--
作者：
Gu, Lingkun;Liu, Yukun;Li, De-Quan
丝裂原激活蛋白激酶（MAPK）级联在植物中发挥着非常重要的作用。它已在模式植物拟南芥、烟草和水稻中得到深入研究。然而，MAPK 在玉米 (Zea mays L.) 中的功能尚未得到充分记录。 ZmSIMK1（玉米盐诱导有丝分裂原激活蛋白激酶 1）是先前在玉米中发现的 MAPK 基因。在本研究中，我们对 ZmSIMK1 进行了表征，并表明 ZmSIMK1 参与拟南芥盐胁迫。分析了 ZmSIMK1 基因的基因组结构及其在玉米中的表达。为了研究ZmSIMK1的功能，我们构建了组成型过表达ZmSIMK1的转基因拟南芥。拟南芥中 ZmSIMK1 的异位表达导致对盐胁迫的抵抗力增强。重要的是，ZmSIMK1 过表达的拟南芥表现出应激反应标记基因 RD29A 和 P5CS1 的组成型表达。此外，RD29A 和 P5CS1 在盐胁迫下表达上调。这些结果表明ZmSIMK1可能在植物盐胁迫中发挥重要作用。
Mitogen-activated protein kinase (MAPK) cascades play a remarkably crucial role in plants. It has been studied intensively in model plants Arabidopsis, tobacco and rice. However, the function of MAPKs in maize (Zea mays L.) has not been well documented. ZmSIMK1 (Zea mays salt-induced mitogen-activated protein kinase 1) is a previously identified MAPK gene in maize. In this research, we charactered ZmSIMK1 and showed that ZmSIMK1 was involved in Arabidopsis salt stress. The genomic organization of ZmSIMK1 gene and its expression in maize have been analyzed. In order to investigate the function of ZmSIMK1, we generated transgenic Arabidopsis constitutively overexpressing ZmSIMK1. Ectopic expression of ZmSIMK1 in Arabidopsis resulted in increased resistance against salt stress. Importantly, ZmSIMK1-overexpressing Arabidopsis exhibited constitutive expression of stress-responsive marker genes, RD29A and P5CS1. Furthermore, RD29A and P5CS1 were upregulated under salt stress. These results suggest that ZmSIMK1 may play an important role in plant salt stress.