A Single-Photon Avalanche Diode Array for Fluorescence Lifetime Imaging Microscopy.
A Single-Photon Avalanche Diode Array for Fluorescence Lifetime Imaging Microscopy.
复制标题
DOI：
10.1109/jssc.2008.2005818
复制
发表时间：
2008-11-21
影响因子：
5.4
通讯作者：
Shepard KL
中科院分区：
文献类型：
--
作者：
Schwartz DE;Charbon E;Shepard KL
我们描述了一个完全集成的单光子雪崩二极管（SPAD）成像器的设计，表征和演示，用于时间分辨荧光成像。该成像器由一个64 × 64的有源SPAD像素阵列和一个基于延迟锁定环（DLL）和校准插值器的片上时间数字转换器（TDC）组成。该成像仪可以执行标准的时间相关单光子计数（TCSPC）和可选的门控窗口检测，用于避免测量亮信号水平时的脉冲堆积。为了说明使用的成像仪，我们提出了测量的几种类型的荧光染料的衰减寿命的定时分辨率为350 ps。
We describe the design, characterization, and demonstration of a fully integrated single-photon avalanche diode (SPAD) imager for use in time-resolved fluorescence imaging. The imager consists of a 64-by-64 array of active SPAD pixels and an on-chip time-to-digital converter (TDC) based on a delay-locked loop (DLL) and calibrated interpolators. The imager can perform both standard time-correlated single-photon counting (TCSPC) and an alternative gated-window detection useful for avoiding pulse pile-up when measuring bright signal levels. To illustrate the use of the imager, we present measurements of the decay lifetimes of fluorescent dyes of several types with a timing resolution of 350 ps.